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PMID: 16912862 Published · ppublish English Journal Article Review

Recent advances in capillary electrophoretic analysis of individual cells.

Analytical and bioanalytical chemistry ·Vol. 387 ·No. 1 ·2007-01-00 ·Pages 51-7

Arcibal IG, Santillo MF, Ewing AG

Abstract

Because variability exists within populations of cells, single-cell analysis has become increasingly important for probing complex cellular environments. Capillary electrophoresis (CE) is an excellent technique for identifying and quantifying the contents of single cells owing to its small volume requirements and fast, efficient separations with highly sensitive detection. Recent progress in both whole-cell and subcellular sampling has allowed researchers to study cellular function in the areas of neuroscience, oncology, enzymology, immunology, and gene expression.

MeSH Terms
Animals Electrophoresis, Capillary/methods,trends Enzymes/analysis,metabolism Eukaryotic Cells/chemistry,metabolism Humans Immunologic Techniques Medical Oncology/methods Neurosciences/methods RNA, Messenger/analysis Subcellular Fractions/chemistry
Chemicals
Enzymes RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Arcibal Imee G
Department of Chemistry, Pennsylvania State University, University Park, PA, USA.
Santillo Michael F
Ewing Andrew G
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Article Info
Journal
Analytical and bioanalytical chemistry
Abbr.
Anal Bioanal Chem
ISSN
1618-2642
DOI
10.1007/s00216-006-0690-0
Published
2007-01-00
Epub
2006-00-16
Pages
51-7
Language
English
Region
Germany
NLM ID
101134327
PMCID
PMC2211411
Subset
IM
Grants
NIGMS NIH HHS · R01 GM078385-02 · United States
External Links
PubMed source DOI: 10.1007/s00216-006-0690-0 PMC Full Text View journal details
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