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PMID: 16912275 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Full and partial genome-wide assembly and disassembly of the yeast transcription machinery in response to heat shock.

Genes & development ·Vol. 20 ·No. 16 ·2006-08-15 ·Pages 2250-65

Zanton SJ, Pugh BF

Abstract

Eukaryotic genes are controlled by sequence-specific DNA-binding proteins, chromatin regulators, general transcription factors, and elongation factors. Here we examine the genome-wide location of representative members of these groups and their redistribution when the Saccharomyces cerevisiae genome is reprogrammed by heat shock. As expected, assembly of active transcription complexes is coupled to eviction of H2A.Z nucleosomes, and disassembly is coupled to the return of nucleosomes. Remarkably, a large number of promoters assemble into partial preinitiation complexes (partial PICs), containing TFIIA, TFIID (and/or SAGA), TFIIB, TFIIE, and TFIIF. However, RNA polymerase II and TFIIH are generally not recruited, and nucleosomes are not displaced. These promoters may be preparing for additional stress that naturally accompany heat stress. For example, we find that oxidative stress, which often occurs with prolonged exposure of cells to high temperature, converts partial PICs into full PICs. Partial PICs therefore represent novel regulated intermediates that assemble at promoters in the midst of chromatin.

MeSH Terms
Chromatin/metabolism Genome, Fungal Heat-Shock Response Histones/metabolism Nucleosomes/metabolism Promoter Regions, Genetic RNA Polymerase II/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
Chromatin HHO1 protein, S cerevisiae Histones Nucleosomes Saccharomyces cerevisiae Proteins Transcription Factors RNA Polymerase II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zanton Sara J
Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Pugh B Franklin
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1437506
Published
2006-08-15
Pages
2250-65
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1553208
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059055 · United States
NIGMS NIH HHS · GM59055 · United States
External Links
PubMed source DOI: 10.1101/gad.1437506 PMC Full Text View journal details
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