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PMID: 1690852 Published · ppublish English Journal Article

The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing.

Molecular and cellular biology ·Vol. 10 ·No. 4 ·1990-04-00 ·Pages 1805-10

Huang MT, Gorman CM

Abstract

Polymerase chain reaction analysis was used to identify aberrant splicing of the simian virus 40 small-t intron present in pRSVcat. We examined factors governing the selection and relative use of aberrant 5' splice sites derived from the chloramphenicol acetyltransferase-coding region. Our results indicated that transcripts from virtually any cDNA positioned upstream of the small-t intron could contain alternative 5' splice sites and therefore be subject to deletions within the protein-coding region.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/genetics Base Sequence Cell Line Genetic Vectors Introns Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction/methods RNA/genetics RNA Splicing Simian virus 40/genetics,immunology Transcription, Genetic Transfection
Chemicals
Antigens, Polyomavirus Transforming Oligonucleotide Probes RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Huang M T
Department of Cell Genetics, Genentech Inc., South San Francisco, California 94080.
Gorman C M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.4.1805-1810.1990
Published
1990-04-00
Pages
1805-10
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362290
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.4.1805-1810.1990 PMC Full Text View journal details
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