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PMID: 16870700 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Novel function of clathrin light chain in promoting endocytic vesicle formation.

Molecular biology of the cell ·Vol. 17 ·No. 10 ·2006-10-00 ·Pages 4343-52

Newpher TM, Idrissi FZ, Geli MI, Lemmon SK

Abstract

Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts with members of the Hip1/R family of endocytic proteins, including the budding yeast homologue, Sla2p. Here, using in vivo analysis in yeast, we provide novel insight into the role of this interaction. We find that overexpression of LC partially restores endocytosis to cells lacking clathrin HC. This suppression is dependent on the Sla2p binding region of LC. Using live cell imaging techniques to visualize endocytic vesicle formation, we find that the N-terminal Sla2p binding region of LC promotes the progression of arrested Sla2p patches that form in the absence of HC. We propose that LC binding to Sla2p positively regulates Sla2p for efficient endocytic vesicle formation.

MeSH Terms
Binding Sites Carrier Proteins/metabolism Clathrin Heavy Chains/genetics Clathrin Light Chains/genetics,physiology Clathrin-Coated Vesicles/physiology Cytoskeletal Proteins Models, Biological Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism,physiology Transport Vesicles/physiology
Chemicals
Carrier Proteins Clathrin Light Chains Cytoskeletal Proteins SLA2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Clathrin Heavy Chains
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Newpher Thomas M
Department of Molecular and Cellular Pharmacology, University of Miami, Miami, FL 33101, USA.
Idrissi Fatima-Zahra
Geli Maria Isabel
Lemmon Sandra K
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-07-0606
Published
2006-10-00
Epub
2006-00-26
Pages
4343-52
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1635359
Subset
IM
Grants
NIGMS NIH HHS · R01 GM055796 · United States
NIGMS NIH HHS · T32 GM008056 · United States
NIGMS NIH HHS · R01 GM55796 · United States
NIGMS NIH HHS · T32 GM08056-20 · United States
External Links
PubMed source DOI: 10.1091/mbc.e06-07-0606 PMC Full Text View journal details
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