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PMID: 16847332 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Requirement of a myocardin-related transcription factor for development of mammary myoepithelial cells.

Molecular and cellular biology ·Vol. 26 ·No. 15 ·2006-08-00 ·Pages 5797-808

Li S, Chang S, Qi X, Richardson JA, Olson EN

Abstract

The mammary gland consists of a branched ductal system comprised of milk-producing epithelial cells that form ductile tubules surrounded by a myoepithelial cell layer that provides contractility required for milk ejection. Myoepithelial cells bear a striking resemblance to smooth muscle cells, but they are derived from a different embryonic cell lineage, and little is known of the mechanisms that control their differentiation. Members of the myocardin family of transcriptional coactivators cooperate with serum response factor to activate smooth muscle gene expression. We show that female mice homozygous for a loss-of-function mutation of the myocardin-related transcription factor A (MRTF-A) gene are unable to effectively nurse their offspring due to a failure in maintenance of the differentiated state of mammary myoepithelial cells during lactation, resulting in apoptosis of this cell population, a consequent inability to release milk, and premature involution. The phenotype of MRTF-A mutant mice reveals a specific and essential role for MRTF-A in mammary myoepithelial cell differentiation and points to commonalities in the transcriptional mechanisms that control differentiation of smooth muscle and myoepithelial cells.

MeSH Terms
Animals Animals, Newborn Body Weight Cell Differentiation Epithelial Cells/cytology,physiology Female In Situ Nick-End Labeling Lactation Mammary Glands, Animal/abnormalities,cytology,growth & development Mice Mice, Inbred C57BL Mice, Knockout Nuclear Proteins/genetics,metabolism Pregnancy Trans-Activators/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
Mrtfa protein, mouse Nuclear Proteins Trans-Activators Transcription Factors myocardin myocardin-related transcription factor B, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Li Shijie
Department of Molecular Biology, The University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., Dallas, TX 75390, USA.
Chang Shurong
Qi Xiaoxia
Richardson James A
Olson Eric N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.00211-06
Published
2006-08-00
Pages
5797-808
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1592772
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.00211-06 PMC Full Text View journal details
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