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PMID: 12397177 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Potentiation of serum response factor activity by a family of myocardin-related transcription factors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 23 ·2002-11-12 ·Pages 14855-60

Wang DZ, Li S, Hockemeyer D, Sutherland L, Wang Z, Schratt G, Richardson JA, Nordheim A, Olson EN

Abstract

Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Blotting, Northern Cloning, Molecular DNA, Complementary Expressed Sequence Tags Gene Expression Regulation, Developmental Glutathione Transferase/genetics Humans Mice Molecular Sequence Data Myocardium/metabolism Nuclear Proteins/genetics,metabolism Recombinant Fusion Proteins/metabolism Sequence Alignment Sequence Homology, Amino Acid Serum Response Factor/genetics,metabolism Trans-Activators/genetics,metabolism Transcription Factors Xenopus
Chemicals
DNA, Complementary Nuclear Proteins Recombinant Fusion Proteins Serum Response Factor Trans-Activators Transcription Factors myocardin Glutathione Transferase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Wang Da-Zhi
Department of Molecular Biology, University of Texas, Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas 75390-9148, USA.
Li Shijie
Hockemeyer Dirk
Sutherland Lillian
Wang Zhigao
Schratt Gerhard
Richardson James A
Nordheim Alfred
Olson Eric N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.222561499
Published
2002-11-12
Epub
2002-00-23
Pages
14855-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC137508
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.222561499 PMC Full Text View journal details
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