Home LiteratureArticle Details
PMID: 1681509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Codon choice and potential complementarity between mRNA downstream of the initiation codon and bases 1471-1480 in 16S ribosomal RNA affects expression of glnS.

Nucleic acids research ·Vol. 19 ·No. 19 ·1991-10-11 ·Pages 5247-51

Faxén M, Plumbridge J, Isaksson LA

Abstract

A cis-acting expression mutation, GAG to GAA, in the third codon of the glnS gene is analyzed. Both codons code for glutamic acid but the mutation is known to increase gene expression by four fold. We show that the mutation has an effect only if it is located in the beginning of a gene but not if located internally. Data are presented that suggest that the reason for the increased expression by the mutation is the potential formation of one more base pair between the mRNA and 16S ribosomal RNA. Gene expression varies about 16 fold as the number of potential base pairs within the sequence 1471-1480 in 16S RNA increase from two to ten. We also give evidence that supports the idea that the presence of rare codons near the beginning of the mRNA can affect expression.

Related Genes
glnS
MeSH Terms
Arginine/genetics Base Composition/genetics Base Sequence Codon/genetics Escherichia coli/genetics Gene Expression Regulation, Bacterial/genetics Glutamate-tRNA Ligase/genetics Glutamates/genetics Glutamic Acid Molecular Sequence Data Mutation/genetics RNA, Messenger/metabolism RNA, Ribosomal, 16S/metabolism Staphylococcal Protein A/genetics Staphylococcus aureus/genetics
Chemicals
Codon Glutamates RNA, Messenger RNA, Ribosomal, 16S Staphylococcal Protein A Glutamic Acid Arginine Glutamate-tRNA Ligase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Faxén M
Department of Microbiology, Stockholm University, Sweden.
Plumbridge J
Isaksson L A
References (25)
25 references, click to expand
  1. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli.
    Gene. 1988 Sep 30;69(2):301-15 PMID: 3069586
  2. Chemical measurement of steady-state levels of ten aminoacyl-transfer ribonucleic acid synthetases in Escherichia coli.
    J Bacteriol. 1977 Jan;129(1):378-87 PMID: 318645
  3. Rates of aminoacyl-tRNA selection at 29 sense codons in vivo.
    J Mol Biol. 1989 Sep 5;209(1):65-77 PMID: 2478714
  4. What constitutes the signal for the initiation of protein synthesis on Escherichia coli mRNAs?
    J Mol Biol. 1988 Nov 5;204(1):79-94 PMID: 2464068
  5. Translation rates of individual codons are not correlated with tRNA abundances or with frequencies of utilization in Escherichia coli.
    J Bacteriol. 1989 Nov;171(11):5812-6 PMID: 2509420
  6. A novel sequence element derived from bacteriophage T7 mRNA acts as an enhancer of translation of the lacZ gene in Escherichia coli.
    J Biol Chem. 1989 Oct 15;264(29):16973-6 PMID: 2676996
  7. Structural basis for misaminoacylation by mutant E. coli glutaminyl-tRNA synthetase enzymes.
    Science. 1989 Dec 1;246(4934):1152-4 PMID: 2686030
  8. Accuracy of in vivo aminoacylation requires proper balance of tRNA and aminoacyl-tRNA synthetase.
    Science. 1988 Dec 16;242(4885):1548-51 PMID: 3144042
  9. A translational enhancer derived from tobacco mosaic virus is functionally equivalent to a Shine-Dalgarno sequence.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):129-32 PMID: 2643095
  10. Characterization of cis-acting mutations which increase expression of a glnS-lacZ fusion in Escherichia coli.
    Mol Gen Genet. 1989 Mar;216(1):113-9 PMID: 2471922
  11. Information content of binding sites on nucleotide sequences.
    J Mol Biol. 1986 Apr 5;188(3):415-31 PMID: 3525846
  12. Secretion of heterologous gene products to the culture medium of Escherichia coli.
    Nucleic Acids Res. 1986 Sep 25;14(18):7487-500 PMID: 3020516
  13. Messenger RNA recognition in Escherichia coli: a possible second site of interaction with 16S ribosomal RNA.
    EMBO J. 1988 Dec 1;7(12):3957-62 PMID: 3061811
  14. A synthetic IgG-binding domain based on staphylococcal protein A.
    Protein Eng. 1987 Feb-Mar;1(2):107-13 PMID: 3507693
  15. Enhancement of translational efficiency by the Escherichia coli atpE translational initiation region: its fusion with two human genes.
    Gene. 1986;41(2-3):201-6 PMID: 3486800
  16. A minor arginine tRNA mutant limits translation preferentially of a protein dependent on the cognate codon.
    J Bacteriol. 1990 May;172(5):2504-10 PMID: 2139647
  17. Suppression of the negative effect of minor arginine codons on gene expression; preferential usage of minor codons within the first 25 codons of the Escherichia coli genes.
    Nucleic Acids Res. 1990 Mar 25;18(6):1465-73 PMID: 2109307
  18. The initiation of translation in E. coli: apparent base pairing between the 16srRNA and downstream sequences of the mRNA.
    Nucleic Acids Res. 1990 Apr 11;18(7):1719-23 PMID: 2186363
  19. Transfer RNA mischarging mediated by a mutant Escherichia coli glutaminyl-tRNA synthetase.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5076-80 PMID: 6382258
  20. Translational initiation in prokaryotes.
    Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
  21. Recognition of messenger RNA during translational initiation in Escherichia coli.
    Biochimie. 1984 Jan;66(1):1-29 PMID: 6370317
  22. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  23. Characterization of translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
  24. The ribosome binding sites recognized by E. coli ribosomes have regions with signal character in both the leader and protein coding segments.
    Nucleic Acids Res. 1980 Sep 11;8(17):3895-907 PMID: 7003539
  25. The AGG codon is translated slowly in E. coli even at very low expression levels.
    Nucleic Acids Res. 1988 Apr 11;16(7):3013-24 PMID: 3285325
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.19.5247
Published
1991-10-11
Pages
5247-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328883
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.19.5247 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2