Home LiteratureArticle Details
PMID: 2643095 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A translational enhancer derived from tobacco mosaic virus is functionally equivalent to a Shine-Dalgarno sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 1 ·1989-01-00 ·Pages 129-32

Gallie DR, Kado CI

Abstract

When present at the 5' end of mRNAs, the untranslated leader sequence (omega) of tobacco mosaic virus RNA significantly enhances translation in eukaryotes and prokaryotes. We have tested a deletion derivative of the omega sequence, omega delta 3, for its enhancing ability on gene constructs in which the ribosomal binding site was either present or deleted, in several Gram-negative bacterial species including Escherichia coli, Agrobacterium tumefaciens, Xanthomonas campestris pv. vitians, Erwinia amylovora, and Salmonella typhimurium. In vivo production of chloramphenicol acetyltransferase from a gene construct lacking its native ribosomal binding site was enhanced 40- to 120-fold by the presence of omega delta 3. Similar levels of enhancement (30- to 240-fold) were observed when the gene encoding beta-glucuronidase was tested. With a chloramphenicol acetyltransferase construct containing a ribosomal binding site, enhancement was markedly less, between 1- and 3.8-fold. Omega delta 3 appeared to enhance translation independent of its position upstream of the AUG codon used for initiation.

MeSH Terms
Amino Acid Sequence Bacteria/genetics Base Sequence Chloramphenicol O-Acetyltransferase/genetics Conjugation, Genetic Enhancer Elements, Genetic Escherichia coli/genetics Molecular Sequence Data Plasmids Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Restriction Mapping Tobacco Mosaic Virus/genetics Transformation, Genetic
Chemicals
RNA, Messenger RNA, Viral Chloramphenicol O-Acetyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gallie D R
Department of Plant Pathology, University of California Davis, 95616.
Kado C I
References (28)
28 references, click to expand
  1. Potential secondary structure at translation-initiation sites.
    Nucleic Acids Res. 1987 Jan 12;15(1):345-60 PMID: 3484332
  2. The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo.
    Nucleic Acids Res. 1987 Apr 24;15(8):3257-73 PMID: 3575095
  3. In vivo uncoating and efficient expression of foreign mRNAs packaged in TMV-like particles.
    Science. 1987 May 29;236(4805):1122-4 PMID: 3472350
  4. Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58.
    J Bacteriol. 1987 Nov;169(11):5101-12 PMID: 2822665
  5. A comparison of eukaryotic viral 5'-leader sequences as enhancers of mRNA expression in vivo.
    Nucleic Acids Res. 1987 Nov 11;15(21):8693-711 PMID: 2825117
  6. Mutational analysis of the tobacco mosaic virus 5'-leader for altered ability to enhance translation.
    Nucleic Acids Res. 1988 Feb 11;16(3):883-93 PMID: 3278300
  7. Characterisation of the 5'-leader sequence of tobacco mosaic virus RNA as a general enhancer of translation in vitro.
    Gene. 1987;60(2-3):217-25 PMID: 2832252
  8. Studies on the mechanism of translational enhancement by the 5'-leader sequence of tobacco mosaic virus RNA.
    Eur J Biochem. 1988 Jul 15;175(1):75-86 PMID: 2841127
  9. The ribosomal fraction mediates the translational enhancement associated with the 5'-leader of tobacco mosaic virus.
    Nucleic Acids Res. 1988 Sep 12;16(17):8675-94 PMID: 3166519
  10. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  11. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
  12. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  13. Autoregulation and function of a repressor in bacteriophage lambda.
    Science. 1976 Oct 8;194(4261):156-61 PMID: 959843
  14. Translation and mRNA decay.
    Mol Gen Genet. 1978 Apr 6;160(2):121-9 PMID: 349350
  15. A general method for maximizing the expression of a cloned gene.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):760-4 PMID: 370836
  16. Operators and promoters in the OR region of phage 434.
    Nucleic Acids Res. 1979 Apr;6(4):1495-508 PMID: 450705
  17. Transformation and preservation of competent bacterial cells by freezing.
    Methods Enzymol. 1979;68:326-31 PMID: 396437
  18. Translational initiation in prokaryotes.
    Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
  19. Construction of a general vector for efficient expression of mammalian proteins in bacteria: use of a synthetic ribosome binding site.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5543-8 PMID: 6272309
  20. Systematic alteration of the nucleotide sequence preceding the translation initiation codon and the effects on bacterial expression of the cloned SV40 small-t antigen gene.
    Gene. 1982 Jan;17(1):55-63 PMID: 6281136
  21. Characterization of translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
  22. Sequences of the Escherichia coli dnaG primase gene and regulation of its expression.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4550-4 PMID: 6750604
  23. Increased synthesis in E. coli of fibroblast and leukocyte interferons through alterations in ribosome binding sites.
    DNA. 1982;1(2):125-31 PMID: 6187521
  24. Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.
    Gene. 1982 Dec;20(2):305-16 PMID: 6299895
  25. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  26. Escherichia coli translational initiation factor IF3: a unique case of translational regulation.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7061-5 PMID: 6390429
  27. The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments.
    Cell. 1986 Jul 18;46(2):245-51 PMID: 3521892
  28. beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447-51 PMID: 3534890
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.1.129
Published
1989-01-00
Pages
129-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286417
Subset
IM
Grants
NCI NIH HHS · CA11526 · United States
Databases
GENBANK
M23043
External Links
PubMed source DOI: 10.1073/pnas.86.1.129 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2