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PMID: 1679335 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Amphetamine-induced and spontaneous release of dopamine from A9 and A10 cell dendrites: an in vitro electrophysiological study in the mouse.

Journal of neural transmission. General section ·Vol. 84 ·No. 3 ·1991-00-00 ·Pages 183-93

Bernardini GL, Gu X, Viscardi E, German DC

Abstract

d-Amphetamine (d-AMP) is a potent releaser of dopamine (DA), and its central nervous system stimulant action is mediated primarily through its effect on the substantia nigra and ventral tegmental area dopaminergic neurons (nuclei A9 and A10, respectively). The purpose of the present experiment was to use electrophysiological techniques to examine dendritic release of DA in the in vitro slice preparation, and determine whether: (1) d-AMP inhibits the firing rates of both A9 and A10 cells; (2) the d-AMP-induced inhibition is mediated via the dendritic release of DA; and (3) there is spontaneous dendritic release of DA. Superfusion with d-AMP (2-100 microM) produced identical inhibitory dose-response curves for A9 and A10 cells, and a dose of 6.25 microM caused more than 50% inhibition in the cell firing rates. The d-AMP-induced inhibition was attenuated by blocking DA synthesis. Either D2 receptor blockade (sulpiride, 1 microM), or DA synthesis inhibition (alpha-methylparatyrosine, 50 microM) resulted in a marked increase in the firing rates of dopaminergic cells. These data suggest that d-AMP comparably releases DA from both A9 and A10 cell dendrites, that it releases newly-synthesized DA to inhibit cell firing, and that DA is tonically released to regulate cell firing rates via interactions with inhibitory D2 autoreceptors.

MeSH Terms
Action Potentials Animals Dendrites/drug effects,metabolism Dextroamphetamine/pharmacology Dopamine/metabolism Dopamine Agents/pharmacology Electrophysiology Male Methyltyrosines/pharmacology Mice Mice, Inbred BALB C Mice, Inbred CBA Receptors, Dopamine/drug effects Secretory Rate/drug effects Substantia Nigra/drug effects,metabolism Sulpiride/pharmacology Synaptic Transmission/drug effects Tegmentum Mesencephali/drug effects,metabolism alpha-Methyltyrosine
Chemicals
Dopamine Agents Methyltyrosines Receptors, Dopamine alpha-Methyltyrosine Sulpiride Dextroamphetamine Dopamine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bernardini G L
Department of Physiology, University of Texas Southwestern Medical Center, Dallas.
Gu X
Viscardi E
German D C
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Article Info
Journal
Journal of neural transmission. General section
Abbr.
J Neural Transm Gen Sect
DOI
10.1007/BF01244969
Published
1991-00-00
Pages
183-93
Language
English
Region
Austria
NLM ID
9002201
Subset
IM
Grants
NIDA NIH HHS · DA-05314 · United States
NIMH NIH HHS · MH-30546 · United States
External Links
PubMed source DOI: 10.1007/BF01244969
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