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PMID: 16738548 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Global analysis of gene function in yeast by quantitative phenotypic profiling.

Molecular systems biology ·Vol. 2 ·2006-00-00 ·Pages 2006.0001

Brown JA, Sherlock G, Myers CL, Burrows NM, Deng C, Wu HI, McCann KE, Troyanskaya OG, Brown JM

Abstract

We present a method for the global analysis of the function of genes in budding yeast based on hierarchical clustering of the quantitative sensitivity profiles of the 4756 strains with individual homozygous deletion of nonessential genes to a broad range of cytotoxic or cytostatic agents. This method is superior to other global methods of identifying the function of genes involved in the various DNA repair and damage checkpoint pathways as well as other interrogated functions. Analysis of the phenotypic profiles of the 51 diverse treatments places a total of 860 genes of unknown function in clusters with genes of known function. We demonstrate that this can not only identify the function of unknown genes but can also suggest the mechanism of action of the agents used. This method will be useful when used alone and in conjunction with other global approaches to identify gene function in yeast.

MeSH Terms
Cluster Analysis DNA Damage/genetics DNA Repair/genetics Gene Deletion Genes, Fungal/physiology Methods Phenotype Saccharomyces cerevisiae/genetics
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Brown James A
Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305-5152, USA. mbrown@stanford.edu
Sherlock Gavin
Myers Chad L
Burrows Nicola M
Deng Changchun
Wu H Irene
McCann Kelly E
Troyanskaya Olga G
Brown J Martin
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Article Info
Journal
Molecular systems biology
Abbr.
Mol Syst Biol
ISSN
1744-4292
DOI
10.1038/msb4100043
Email
mbrown@stanford.edu
Published
2006-00-00
Epub
2006-00-17
Pages
2006.0001
Language
English
Region
England
NLM ID
101235389
PMCID
PMC1681475
Subset
IM
Grants
NCI NIH HHS · T32 CA009302 · United States
NCI NIH HHS · P01 CA067166 · United States
NIGMS NIH HHS · R01 GM071966 · United States
NCI NIH HHS · P01 CA67166 · United States
NHGRI NIH HHS · R01 HG003469 · United States
NIGMS NIH HHS · 1R01GM071966-01A1 · United States
NHGRI NIH HHS · T32 HG003284-01 · United States
NHGRI NIH HHS · T32 HG003284 · United States
NCI NIH HHS · CA09302 · United States
External Links
PubMed source DOI: 10.1038/msb4100043 PMC Full Text View journal details
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