Phosphoinositides in barley aleurone layers and gibberellic Acid-induced changes in metabolism.

Plant physiology ·Vol. 91 ·No. 4 ·1989-12-00 ·Pages 1266-9

Murthy PP, Renders JM, Keranen LM

Abstract

Phospholipids of barley (Hordeum vulgare L. cv Himalaya) aleurone layers were labeled with myo-[2-(3)H]inositol or [(32)Pi], extracted, and analyzed by physical (chromatography) and chemical (deacylation) techniques. Three phospholipids were found to incorporate both myo-[2-(3)H]inositol and [(32)Pi]-phosphatidylinositol, phosphatidylinositol-monophosphate, and phosphatidylinositol-bisphosphate. Stimulation of [(3)H]inositol prelabeled aleurone layers with GA(3) showed enhanced incorporation of label into phosphatidylinositol within 30 seconds and subsequent rapid breakdown. Stimulation of phosphatidylinositol labeling observed in these studies is the earliest response of aleurone cells to gibberellic acid reported.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Murthy P P

Department of Chemistry and Chemical Engineering, Michigan Technological University, Houghton, Michigan 49931.

Renders J M

Keranen L M

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15 references, click to expand

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.91.4.1266

Published

1989-12-00

Pages

1266-9

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC1062177

External Links

PubMed source DOI: 10.1104/pp.91.4.1266 PMC Full Text View journal details

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Phosphoinositides in barley aleurone layers and gibberellic Acid-induced changes in metabolism.

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