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PMID: 1664711 Published · ppublish English Journal Article

Phage abortive infection mechanism from Lactococcus lactis subsp. lactis, expression of which is mediated by an Iso-ISS1 element.

Applied and environmental microbiology ·Vol. 57 ·No. 12 ·1991-12-00 ·Pages 3547-51

Cluzel PJ, Chopin A, Ehrlich SD, Chopin MC

Abstract

A 5-kb DNA fragment conferring a phage abortive infection phenotype (Abi+) has been cloned from Lactococcus lactis subsp. lactis IL416. The Abi+ determinant was subcloned on a 2-kb fragment which carried an Iso-ISS1 element and an open reading frame of 753 bp designated ORFX. Deletion within ORFX entailed the loss of the Abi+ phenotype, establishing that ORFX is the structural abi-416 gene. The expression of abi-416 was shown to be mediated by the Iso-ISS1 element, which contains a sequence fitting the consensus sequence for gram-positive promoters.

Related Genes
abi-416
MeSH Terms
Amino Acid Sequence Bacteriophages/physiology Base Sequence Cloning, Molecular DNA Transposable Elements DNA, Bacterial Gene Expression Regulation, Bacterial Genes, Bacterial Kinetics Lactococcus lactis/genetics Molecular Sequence Data Nucleotidyltransferases/chemistry,genetics Open Reading Frames Phenotype Promoter Regions, Genetic Transcription, Genetic Transposases
Chemicals
DNA Transposable Elements DNA, Bacterial Nucleotidyltransferases Transposases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cluzel P J
Laboratoire de Génétique Microbienne, Institut de Biotechnologie, I.N.R.A., Jouy-en-Josas, France.
Chopin A
Ehrlich S D
Chopin M C
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.57.12.3547-3551.1991
Published
1991-12-00
Pages
3547-51
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC184010
Subset
IM
Databases
GENBANK
M63190, M63191, M63292, M63293, M63294, M63295, M77708, S77323, S77325, S80807
External Links
PubMed source DOI: 10.1128/aem.57.12.3547-3551.1991 PMC Full Text View journal details
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