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PMID: 2508557 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

DNA-DNA homology among lactose- and sucrose-fermenting transconjugants from Lactococcus lactis strains exhibiting reduced bacteriophage sensitivity.

Applied and environmental microbiology ·Vol. 55 ·No. 9 ·1989-09-00 ·Pages 2410-3

Steele JL, Murphy MC, Daly C, McKay LL

Abstract

DNA-DNA homology between a reduced bacteriophage sensitivity (Rbs+) probe and DNA from both Rbs+ and Rbs- Lactococcus lactis strains was examined. Homology was detected between the probe and five plasmids (pCI750, pCC34, pEB56, pNP2, and pJS88) isolated from lactose-positive Rbs+ transconjugants and between the probe and genomic DNA of a sucrose-positive Rbs+ transconjugant. Additionally, hybridizations conducted between the probe and plasmids reported to encode abortive bacteriophage infection indicated homology with pTR2030 but not with pBF61 and pGBK17. The results suggest that a common genetic determinant(s) may be present in a variety of lactococcal plasmids coding for Rbs+.

MeSH Terms
Bacteriophages/genetics Base Sequence Conjugation, Genetic DNA, Bacterial/genetics Fermentation Lactococcus lactis/genetics,metabolism Lactose/metabolism Phenotype Sequence Homology, Nucleic Acid Sucrose/metabolism
Chemicals
DNA, Bacterial Sucrose Lactose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Steele J L
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.
Murphy M C
Daly C
McKay L L
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.55.9.2410-2413.1989
Published
1989-09-00
Pages
2410-3
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC203090
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.55.9.2410-2413.1989 PMC Full Text View journal details
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