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PMID: 1661735 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two alternatively spliced mouse urokinase receptor mRNAs with different histological localization in the gastrointestinal tract.

The Journal of cell biology ·Vol. 115 ·No. 6 ·1991-12-00 ·Pages 1763-71

Kristensen P, Eriksen J, Blasi F, Danø K

Abstract

Two mouse urokinase-type plasminogen activator receptor (muPAR) cDNAs were isolated: muPAR1 is homologous to the human urokinase-type plasminogen activator receptor while muPAR2 codes for a 199 residue protein sharing the first 133 residues with muPAR1. Mouse genomic DNA sequencing indicates that the two different mRNAs arise by alternative splicing. In situ hybridization showed differential expression of the two mRNAs in mouse gastric mucosa. muPAR1 mRNA is located in luminal epithelial cells situated close to urokinase-type plasminogen activator-producing connective tissue cells of the lamina propria, pointing to plasmin generation controlled by the cooperation of different cells that may play a role in the release of gastric epithelial cells. muPAR2 mRNA is expressed in the basal epithelial cells, and the deduced protein sequence includes the receptor ligand binding domain, but omits the region involved in glycolipid-mediated membrane anchoring, suggesting that muPAR2 may code for a secreted uPA binding protein.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA/isolation & purification Gastric Mucosa/metabolism Humans Mice Molecular Sequence Data Nucleic Acid Hybridization Protein Biosynthesis RNA Splicing RNA, Messenger/metabolism Receptors, Cell Surface/genetics,metabolism Receptors, Urokinase Plasminogen Activator Sequence Alignment Sequence Homology, Nucleic Acid Urokinase-Type Plasminogen Activator/metabolism
Chemicals
PLAUR protein, human Plaur protein, mouse RNA, Messenger Receptors, Cell Surface Receptors, Urokinase Plasminogen Activator DNA Urokinase-Type Plasminogen Activator
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kristensen P
Finsen Laboratory, Rigshospitalet, Copenhagen, Denmark.
Eriksen J
Blasi F
Danø K
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.115.6.1763
Published
1991-12-00
Pages
1763-71
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289218
Subset
IM
Databases
GENBANK
M60692, M60693, M60694, M60695, M60696, M60697, S66758, S66760, S66765, X62700, X62701
External Links
PubMed source DOI: 10.1083/jcb.115.6.1763 PMC Full Text View journal details
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