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PMID: 16606668 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of an interleukin (IL)-25-dependent cell population that provides IL-4, IL-5, and IL-13 at the onset of helminth expulsion.

The Journal of experimental medicine ·Vol. 203 ·No. 4 ·2006-04-17 ·Pages 1105-16

Fallon PG, Ballantyne SJ, Mangan NE, Barlow JL, Dasvarma A, Hewett DR, McIlgorm A, Jolin HE, McKenzie AN

Abstract

Type 2 immunity, which involves coordinated regulation of innate and adaptive immune responses, can protect against helminth parasite infection, but may lead to allergy and asthma after inappropriate activation. We demonstrate that il25(-/-) mice display inefficient Nippostrongylus brasiliensis expulsion and delayed cytokine production by T helper 2 cells. We further establish a key role for interleukin (IL)-25 in regulating a novel population of IL-4-, IL-5-, IL-13-producing non-B/non-T (NBNT), c-kit+, FcepsilonR1- cells during helminth infection. A deficit in this population in il25(-/-) mice correlates with inefficient N. brasiliensis expulsion. In contrast, administration of recombinant IL-25 in vivo induces the appearance of NBNT, c-kit+, FcepsilonR1- cells and leads to rapid worm expulsion that is T and B cell independent, but type 2 cytokine dependent. We demonstrate that these IL-25-regulated cells appear rapidly in the draining lymph nodes, implicating them as a source of type 2 cytokines during initiation of worm expulsion.

MeSH Terms
Animals B-Lymphocytes/cytology,immunology,metabolism Basophils/metabolism Cells, Cultured Cytokines/biosynthesis,classification Interleukin-13/biosynthesis,deficiency,genetics Interleukin-4/biosynthesis,deficiency,genetics Interleukin-5/biosynthesis,deficiency,genetics Interleukins/administration & dosage,deficiency,genetics,physiology Mice Mice, Inbred BALB C Mice, Knockout Nippostrongylus/immunology Proto-Oncogene Proteins c-kit/metabolism Receptors, IgE/deficiency Recombinant Proteins/administration & dosage,genetics,metabolism Strongylida Infections/immunology,parasitology T-Lymphocytes/cytology,immunology,metabolism
Chemicals
Cytokines Interleukin-13 Interleukin-5 Interleukins Mydgf protein, mouse Receptors, IgE Recombinant Proteins Interleukin-4 Proto-Oncogene Proteins c-kit
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Fallon Padraic G
Department of Biochemistry, Trinity College, Dublin 2, Ireland.
Ballantyne Sarah J
Mangan Niamh E
Barlow Jillian L
Dasvarma Ayan
Hewett Duncan R
McIlgorm Ann
Jolin Helen E
McKenzie Andrew N J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20051615
Published
2006-04-17
Epub
2006-00-10
Pages
1105-16
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118283
Subset
IM
Grants
Medical Research Council · MC_U105178805 · United Kingdom
Corrections
CommentIn
PMID: 16606679
External Links
PubMed source DOI: 10.1084/jem.20051615 PMC Full Text View journal details
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