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PMID: 16592326 Published · ppublish English Journal Article

Transcriptional origin of Euglena chloroplast tRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 6 ·1976-06-00 ·Pages 1984-8

Schwartzbach SD, Hecker LI, Barnett WE

Abstract

tRNA.DNA hybridization studies indicate that Euglena chloroplast tRNAs are transcriptional products of the chloroplast genome, which contains approximately 26 tRNA cistrons. Hybridization with purified chloroplast tRNA(Phe) and tRNA(Asp) shows that the chloroplast genome contains one cistron for each of these two species. No hybridization of chloroplast tRNA with nuclear DNA was observed. tRNAs from Euglena cytoplasm, Escherichia coli, and Agmenellum quadraduplicatum do not compete with chloroplast tRNA for hybridization with chloroplast DNA. Evidence is presented that photoinduction of chloroplast tRNAs is at the level of transcription rather than maturation of tRNA precursor molecules.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schwartzbach S D
Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830.
Hecker L I
Barnett W E
References (25)
25 references, click to expand
  1. Ribosomal RNA cistrons in Euglena gracilis.
    J Mol Biol. 1973 Dec 15;81(3):327-36 PMID: 4203370
  2. Identification and origin of some chloroplast aminoacyl-tRNA synthetases and tRNAs.
    Proc Natl Acad Sci U S A. 1970 Nov;67(3):1207-13 PMID: 5274450
  3. Isolation and molecular weight of circular chloroplast DNA from Euglena gracilis.
    Biochim Biophys Acta. 1972 Feb 15;259(3):285-96 PMID: 4622384
  4. STUDIES OF CHLOROPLAST DEVELOPMENT IN EUGLENA. XII. TWO TYPES OF SATELLITE DNA.
    J Mol Biol. 1965 Apr;11:769-74 PMID: 14338786
  5. Isolation and partial characterization of the transfer ribonucleic acid cistrons from Escherichia coli.
    Nature. 1970 Aug 1;227(5257):448-51 PMID: 4913717
  6. The number of 4-S RNA genes on yeast mitochondrial DNA.
    Biochem Biophys Res Commun. 1972 Apr 14;47(1):126-33 PMID: 4554811
  7. Chloroplast ribosomal RNA genes in the chloroplast DNA of Euglena gracilis.
    J Mol Biol. 1973 Jun 15;77(1):125-32 PMID: 4203658
  8. Expression of the mitochondrial genome in HeLa cells. XIV. The relative positions of the 4 S RNA genes and of the ribosomal RNA genes in mitochondrial DNA.
    J Mol Biol. 1972 Oct 28;71(1):81-93 PMID: 4634988
  9. Expression of the mitochondria genome in HeLa cells. IV. Titration of mitochondrial genes for 16 s, 12 s and 4 s RNA.
    J Mol Biol. 1971 Jan 28;55(2):271-6 PMID: 5548609
  10. Mitochondrial RNA In Xenopus laevis. I. The expression of the mitochondrial genome.
    J Mol Biol. 1972 Jan 28;63(2):201-16 PMID: 4564177
  11. Total synthesis of the gene for an alanine transfer ribonucleic acid from yeast.
    Nature. 1970 Jul 4;227(5253):27-34 PMID: 5422620
  12. The sites of transcription and translation for Euglena chloroplastic aminoacyl-tRNA synthetases.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1910-4 PMID: 4525469
  13. Characteristics of a stable, filamentous mutant of a coccoid blue-green alga.
    J Bacteriol. 1970 Jun;102(3):784-9 PMID: 4988043
  14. Comparative base compositions of chloroplast and cytoplasmic tRNAPhe's from Euglena gracilis.
    Nucleic Acids Res. 1976 Feb;3(2):371-80 PMID: 815890
  15. Hybridization of leucyl-transfer ribonucleic Acid isoacceptors from green leaves with nuclear and chloroplast deoxyribonucleic Acid.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12 Pt 1-2):3498-501 PMID: 16592122
  16. The kinetic complexity of Euglena gracilis chloroplast DNA.
    FEBS Lett. 1975 Apr 1;52(2):171-4 PMID: 805712
  17. Partial characterization of transfer RNA genes isolated from Neurospora crassa.
    Mol Gen Genet. 1973 Sep 27;125(4):295-300 PMID: 4273106
  18. The effect of genetic complexity on the time-course of ribonucleic acid-deoxyribonucleic acid hybridization.
    Biochem J. 1969 Aug;113(5):805-11 PMID: 4898022
  19. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
    J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314
  20. Iodination of nucleic acids in vitro.
    Biochemistry. 1971 May 25;10(11):1993-2000 PMID: 4935105
  21. Induction of euglena transfer RNA's by light.
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1261-8 PMID: 5260929
  22. The chloroplast and cytoplasmic ribosomes of euglena: I. Stability of chloroplast ribosomes prepared by an improved procedure.
    Plant Physiol. 1974 Apr;53(4):533-42 PMID: 16658740
  23. The influence of chain length and base composition on the specific association of oligoribonucleotides with denatured deoxyribonucleic acid.
    J Biol Chem. 1969 Mar 25;244(6):1576-81 PMID: 5773060
  24. Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
    J Mol Biol. 1968 Jun 28;34(3):661-80 PMID: 5760467
  25. Ribosomal DNA satellite of Euglena gracilis chloroplast DNA.
    FEBS Lett. 1971 Oct 1;17(2):277-280 PMID: 11946046
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.6.1984
Published
1976-06-00
Pages
1984-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430432
External Links
PubMed source DOI: 10.1073/pnas.73.6.1984 PMC Full Text View journal details
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