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PMID: 16557984 Published · ppublish English Journal Article

Group a streptococcal polysaccharide antigens.

Infection and immunity ·Vol. 3 ·No. 3 ·1971-03-00 ·Pages 385-9

Matsuno T, Slade HD

Abstract

Cold trichloroacetic acid extracts of group A, type 3 streptococci were fractionated on Sephadex G50 and ECTEOLA cellulose columns. A glucose-N-acetylglucosamine polymer was isolated and shown by chemical, electrophoretic, and antigenic analysis to be distinct from the group A polysaccharide. The antigenic specificity was due to the glucosamine. A glycerol-phosphate-alanine-glucosamine teichoic acid polymer was isolated. The antigenic specificity was due to N-acetylglucosamine and d-alanine methyl ester, with the former possessing principal responsibility. The glucosamine and ester-linked alanine appear to be labile to the method of extraction and fractionation. Group A streptococcal whole cell antiserum, after adsorption with cells of a heterologous serological group and group A polysaccharide antigen, contained antibodies against both polymers.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Matsuno T
Department of Microbiology, Northwestern University Medical School, Chicago, Illinois 60611.
Slade H D
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.3.3.385-389.1971
Published
1971-03-00
Pages
385-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC416162
External Links
PubMed source DOI: 10.1128/iai.3.3.385-389.1971 PMC Full Text View journal details
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