Rapid fluorescent-antibody conjugation procedure.

Infection and immunity ·Vol. 3 ·No. 2 ·1971-02-00 ·Pages 355-7

Peacock M, Burgdorfer W, Ormsbee RA

Abstract

This note describes a technique developed for rapid preparation of specific and brilliant conjugates for direct staining of rickettsial agents in yolk-sac, tick, and vertebrate tissues.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Peacock M

U. S. Department of Health, Education, and Welfare, Public Health Service, Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.

Burgdorfer W

Ormsbee R A

References (10)

10 references, click to expand

The use of fluorescent antibody in the diagnosis and study of viral and rickettsial infections.
Ergeb Mikrobiol Immunitatsforsch Exp Ther. 1960;33:242-58 PMID: 14417598
A PREPARATIVE METHOD FOR THE SEPARATION OF 7S GAMMA GLOBULIN FROM HUMAN SERUM.
Arch Biochem Biophys. 1964 Dec;108:514-22 PMID: 14244695
FACTORS AFFECTING THE RATE OF REACTION OF FLUORESCEIN ISOTHIOCYANATE WITH SERUM PROTEINS.
J Immunol. 1964 Aug;93:232-42 PMID: 14202251
Ultra-rapid fluorescent labelling of proteins.
Nature. 1962 Jan 13;193:167-8 PMID: 14492332
The chromatographic purification of fluorescein-antibody.
J Histochem Cytochem. 1961 Sep;9:484-6 PMID: 13882753
Fluorescent antibody methods.
Gen Cytochem Methods. 1958;1:399-422 PMID: 13574437
A microagglutination technique for detection and measurement of rickettsial antibodies.
Acta Virol. 1969 Jan;13(1):60-6 PMID: 4387631
Direct immunofluorescent tissue culture assay for lymphocytic choriomeningitis virus.
J Immunol. 1968 May;100(5):1135-8 PMID: 4967592
Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method.
Immunology. 1970 Jun;18(6):875-81 PMID: 4913804
Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
Immunology. 1970 Jun;18(6):865-73 PMID: 5310665

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.3.2.355-357.1971

Published

1971-02-00

Pages

355-7

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC416155

External Links

PubMed source DOI: 10.1128/iai.3.2.355-357.1971 PMC Full Text View journal details

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