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PMID: 5310665 Published · ppublish English Journal Article

Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.

Immunology ·Vol. 18 ·No. 6 ·1970-06-00 ·Pages 865-73

The TH, Feltkamp TE

Abstract

暂无摘要

MeSH Terms
Animals Antibodies Antibodies, Anti-Idiotypic Chromatography, DEAE-Cellulose Fluoresceins Horses Humans Hydrogen-Ion Concentration Immunoglobulin G Protein Binding Rabbits Sheep Temperature Thiocyanates
Chemicals
Antibodies Antibodies, Anti-Idiotypic Fluoresceins Immunoglobulin G Thiocyanates
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
The T H
Feltkamp T E
References (17)
17 references, click to expand
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  7. A provisional specification for fluorescein-labelled antibody against human globulin (IgG).
    Mon Bull Minist Health Public Health Lab Serv. 1967 Sep;26:179-81 PMID: 4866889
  8. Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method.
    Immunology. 1970 Jun;18(6):875-81 PMID: 4913804
  9. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera.
    J Exp Med. 1951 Feb;93(2):107-20 PMID: 14803635
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    Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898-900 PMID: 13591380
  11. Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins.
    J Exp Med. 1961 Jul 1;114:89-110 PMID: 13706641
  12. A kinetic study on the labeling of serum globulin with fluorescein isothiocyanate by means of the gel filtration technique.
    J Immunol. 1962 Aug;89:195-203 PMID: 13921593
  13. PURIFICATION AND ANALYSIS OF FLUORESCEIN-LABELED ANTISERA BY COLUMN CHROMATOGRAPHY.
    J Immunol. 1963 Apr;90:634-42 PMID: 14082026
  14. FACTORS AFFECTING THE RATE OF REACTION OF FLUORESCEIN ISOTHIOCYANATE WITH SERUM PROTEINS.
    J Immunol. 1964 Aug;93:232-42 PMID: 14202251
  15. Two different anti-insulin antibodies in guinea pig antisera.
    J Immunol. 1962 Sep;89:442-51 PMID: 14008707
  16. SEPARATION OF FLUORESCENT ANTIBODY CONJUGATES INTO 7S AND 19S GLOBULIN COMPONENTS BY GEL FILTRATION.
    Proc Soc Exp Biol Med. 1965 Jul;119:748-51 PMID: 14328994
  17. Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies.
    J Immunol. 1965 Aug;95(2):225-9 PMID: 4158327
Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1970-06-00
Pages
865-73
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1455721
Subset
IM
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