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PMID: 16535064 Published · ppublish English Journal Article

Expression of a Lactococcus lactis Phage Resistance Mechanism by Streptococcus thermophilus.

Applied and environmental microbiology ·Vol. 61 ·No. 7 ·1995-07-00 ·Pages 2461-6

Moineau S, Walker SA, Holler BJ, Vedamuthu ER, Vandenbergh PA

Abstract

The 7.8-kb lactococcal plasmid pSRQ700 encodes the LlaII restriction/modification system which recognizes and cleaves the sequence 3(prm1)-GATC-5(prm1). When the plasmid pSRQ700 is introduced into a phage-sensitive Lactococcus lactis strain, strong phage resistance is conferred by the LlaII system. In this report, we show that pSRQ700 cannot replicate in Streptococcus thermophilus. However, if cloned into the vector pNZ123, the native LlaII system is expressed and strong phage resistance is conferred to various industrial S. thermophilus strains. Resistance against phages isolated from yogurt and mozzarella wheys was observed. To our knowledge, this is the first report of increased phage resistance in S. thermophilus.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moineau S
Walker S A
Holler B J
Vedamuthu E R
Vandenbergh P A
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30 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.61.7.2461-2466.1995
Published
1995-07-00
Pages
2461-6
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC1388482
External Links
PubMed source DOI: 10.1128/aem.61.7.2461-2466.1995 PMC Full Text View journal details
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