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PMID: 16349277 Published · ppublish English Journal Article

Evolution of a Lytic Bacteriophage via DNA Acquisition from the Lactococcus lactis Chromosome.

Applied and environmental microbiology ·Vol. 60 ·No. 6 ·1994-06-00 ·Pages 1832-41

Moineau S, Pandian S, Klaenhammer TR

Abstract

We discovered a phage-host interaction in which the lytic phage ul36, in response to pressure exerted by an abortive phage resistance mechanism, acquired a large DNA fragment from the chromosome of Lactococcus lactis NCK203 to form a new phage, ul37. Phage ul37 was characterized at morphological, phenotypic, and genotypic levels and was found to be a member of the P335 species. Although it exhibits a high level of DNA homology with ul36, phage ul37 is resistant to the abortive mechanism and has a longer tail, a different base plate, and apparently a different origin of replication. The chromosomal DNA implicated in the formation of new phage ul37 was disrupted by site-specific integration in NCK203. This strategy prevented the appearance of ul37 during subsequent infections with ul36.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Moineau S
Department of Food Science and Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, North Carolina 27695-7624.
Pandian S
Klaenhammer T R
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.60.6.1832-1841.1994
Published
1994-06-00
Pages
1832-41
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC201570
External Links
PubMed source DOI: 10.1128/aem.60.6.1832-1841.1994 PMC Full Text View journal details
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