Construction of a Bacteriophage-Resistant Derivative of Lactococcus lactis subsp. lactis 425A by Using the Conjugal Plasmid pNP40.

Applied and environmental microbiology ·Vol. 57 ·No. 12 ·1991-12-00 ·Pages 3405-9

Harrington A, Hill C

Abstract

Lactococcus lactis subsp. lactis 425A is an atypical strain which excretes a high concentration of alpha-acetolactate when grown in milk. The conjugative lactococcal plasmid pNP40, which encodes phage and nisin resistance, was introduced to strain 425A by conjugation, using resistance to phage and nisin as a selection. No phage-nisin resistance mutants were encountered. Transconjugants display complete resistance at both 21 and 39 degrees C to those phage previously identified as lytic for 425A. Transconjugants lose their resistance characteristics when spontaneously cured of pNP40. The commercially important property of 425A-production of high levels of alpha-acetolactic acid-is unaffected by the presence of pNP40.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Harrington A

The National Dairy Products Research Centre, Moorepark, Fermoy, County Cork, Irish Republic.

Hill C

References (12)

12 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.57.12.3405-3409.1991

Published

1991-12-00

Pages

3405-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC183987

External Links

PubMed source DOI: 10.1128/aem.57.12.3405-3409.1991 PMC Full Text View journal details

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Construction of a Bacteriophage-Resistant Derivative of Lactococcus lactis subsp. lactis 425A by Using the Conjugal Plasmid pNP40.

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