Home LiteratureArticle Details
PMID: 165169 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of mutants of Haemophilus influenzae deficient in an adenosine 5'-triphosphate-dependent deoxyribonuclease activity.

Journal of bacteriology ·Vol. 122 ·No. 2 ·1975-05-00 ·Pages 443-53

Wilcox KW, Smith HO

Abstract

By a direct assay approach, mutants of Haemophilus influenzae Rd that are deficient in adenosine 5'-triphosphate-dependent deoxyribonuclease activity (add-) were isolated and characterized. A large proportion (50 to 90%) of the cells in cultures of these mutants failed to produce visible colonies when plated. An extensive analysis of the recombination proficiency of these strains revealed that the transformation frequency (transformants per competent cell) in the mutants was similar to that found in the wild type, but that the transformation efficiency (transformants per microgram of irreversibly bound deoxyribonucleic acid [DNA]) was reduced approximately fourfold. Sensitivities of the mutants to gamma rays, ultraviolet radiation, and methyl methane sulfonate were only slightly greater than wild-type levels. The rate of degradation of host DNA after ultraviolet irradiation was significantly reduced in the mutants. It is suggested that the adenosine 5'-triphosphate-dependent deoxyribonuclease in H. influenzae plays a nonessential role in DNA recombination and repair.

MeSH Terms
Adenosine Triphosphate/metabolism Autoradiography Cell-Free System Cesium Radioisotopes DNA Repair DNA, Bacterial/metabolism,radiation effects Deoxyribonucleases/metabolism Haemophilus influenzae/enzymology,metabolism,radiation effects Mesylates/pharmacology Mutation Radiation Effects Recombination, Genetic Transformation, Genetic Ultraviolet Rays
Chemicals
Cesium Radioisotopes DNA, Bacterial Mesylates Adenosine Triphosphate Deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wilcox K W
Smith H O
References (29)
29 references, click to expand
  1. Adenosine triphosphate-dependent deoxyribonuclease from Diplococcus pneumoniae: fate of transforming deoxyribonucleic acid in a strain deficient in the enzymatic activity.
    J Bacteriol. 1973 Feb;113(2):718-23 PMID: 4144144
  2. ATP-dependent deoxyribonuclease in Bacillus subtilis and a mutant deficient in this activity.
    Mutat Res. 1974 Jan;22(1):15-23 PMID: 4210325
  3. An adenosine triphosphate-dependent deoxyribonuclease from Hemophilus influenzae Rd. II. Adenosine triphosphatase properties.
    J Biol Chem. 1972 May 10;247(9):2854-8 PMID: 4260215
  4. An adenosine triphosphate-dependent deoxyribonuclease from Hemophilus influenzae Rd. 3. Substrate specificity.
    J Biol Chem. 1972 May 10;247(9):2859-65 PMID: 4337105
  5. Mutants of Escherichia coli with altered deoxyribonucleases. I. Isolation and characterization of mutants for exonuclease 3.
    J Mol Biol. 1972 Jul 21;68(2):303-18 PMID: 4341749
  6. Studies on ATP-dependent deoxyribonuclease of Haemophilus influenzae: involvement of the enzyme in the transformation process.
    Biochem Biophys Res Commun. 1973 Sep 5;54(1):1-8 PMID: 4354944
  7. Number of transformable units per cell in Diplococcus pneumoniae.
    J Bacteriol. 1969 Mar;97(3):1033-5 PMID: 4388384
  8. An ATP-dependent deoxyribonuclease from Diplococcus pneumoniae. II. Evidence for its involvement in bacterial recombination.
    Biochim Biophys Acta. 1970 Nov 12;224(1):42-54 PMID: 4395244
  9. An adenosine triphosphate-dependent deoxyribonuclease from Hemophilus influenzae Rd. I. Purification and properties of the enzyme.
    J Biol Chem. 1972 May 10;247(9):2846-53 PMID: 4554362
  10. Production of possible recombination intermediates by an ATP-dependent DNAase.
    Nat New Biol. 1973 Jan 10;241(106):54-8 PMID: 4572840
  11. Specificity in deoxyribonucleic acid uptake by transformable Haemophilus influenzae.
    J Bacteriol. 1974 May;118(2):369-73 PMID: 4597440
  12. Some properties of ATP dependent deoxyribonucleases from normal and rec-mutant strains of Bacillus subtilis.
    FEBS Lett. 1972 Jul 15;24(1):121-5 PMID: 4628739
  13. DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes.
    Radiat Res. 1966;:Suppl 6:156+ PMID: 5334390
  14. Studies on transformations of Hemophilus influenzae. I. Competence.
    J Gen Physiol. 1961 Jul;44:1201-27 PMID: 13707010
  15. A DEOXYRIBONUCLEASE REACTION REQUIRING NUCLEOSIDE DI- OR TRIPHOSPHATES.
    Biochemistry. 1964 Nov;3:1678-84 PMID: 14235329
  16. Competence Mutant of Haemophilus influenzae with Abnormal Ratios of Marker Efficiencies in Transformation.
    J Bacteriol. 1972 Oct;112(1):492-502 PMID: 16559162
  17. DNA repair.
    Annu Rev Biochem. 1968;37:175-200 PMID: 4875714
  18. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.
    J Bacteriol. 1969 Jan;97(1):244-9 PMID: 4884815
  19. Enzymatic DNA degradation in E. coli: its relationship to synthetic processes at the chromosome level.
    Cold Spring Harb Symp Quant Biol. 1968;33:259-69 PMID: 4891968
  20. Spontaneous lethal sectoring, a further feature of Escherichia coli strains deficient in the function of rec and uvr genes.
    J Bacteriol. 1968 Sep;96(3):652-9 PMID: 4895049
  21. [Mechanisms of enzyme degradation of bacterial chromosomes and their regulation].
    Bull Soc Chim Biol (Paris). 1969;51(10):1373-83 PMID: 4906603
  22. An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination.
    Proc Natl Acad Sci U S A. 1969 Dec;64(4):1292-9 PMID: 4916924
  23. Toward a metabolic interpretation of genetic recombination of E. coli and its phages.
    Annu Rev Microbiol. 1971;25:437-64 PMID: 4949037
  24. Repair of deoxyribonucleic acid in Haemophilus influenzae. I. X-ray sensitivity of ultraviolet-sensitive mutants and their behavior as hosts to ultraviolet-irradiated bacteriophage and transforming deoxyribonucleic acid.
    J Bacteriol. 1968 Feb;95(2):546-58 PMID: 5300300
  25. Defined medium for growth of Haemophilus influenzae.
    J Bacteriol. 1970 Feb;101(2):513-6 PMID: 5308770
  26. Competence mutants: isolation of transformation deficient strains of Haemophilus influenzae.
    Nature. 1970 Aug 1;227(5257):515-7 PMID: 5310646
  27. Fate of donor DNA in some poorly transformable strains of Haemophilus influenzae.
    Mutat Res. 1970 Mar;9(3):245-53 PMID: 5312331
  28. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
    J Mol Biol. 1970 Jul 28;51(2):379-91 PMID: 5312500
  29. Regulation of chromosome replication in Escherichia coli: alternate replication of two chromosomes at slow growth rates.
    J Mol Biol. 1965 Aug;13(1):105-26 PMID: 5323611
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.122.2.443-453.1975
Published
1975-05-00
Pages
443-53
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246077
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.122.2.443-453.1975 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2