Defined medium for growth of Haemophilus influenzae.

Journal of bacteriology ·Vol. 101 ·No. 2 ·1970-02-00 ·Pages 513-6

Herriott RM, Meyer EY, Vogt M, Modan M

Abstract

A chemically defined medium (MI(c)) is described in which cells of Haemophilus influenzae Rd grow rapidly (generation time, 35 +/- 5 min) and reach a stationary level of 10(10) cells/ml. Our strain of cells grown in this medium developed high levels of competence when transferred to another medium designed for that purpose. Conditions governing the total development of competence in this organism have now been defined.

MeSH Terms

Amino Acids Culture Media DNA, Bacterial/metabolism Haemophilus influenzae/growth & development Nucleosides Phosphorus Isotopes Time Factors

Chemicals

Amino Acids Culture Media DNA, Bacterial Nucleosides Phosphorus Isotopes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Herriott R M

Meyer E Y

Vogt M

Modan M

References (10)

10 references, click to expand

Inosine and lactate: factors critical during growth for development of competence in Haemophilus influenzae.
Biochem Biophys Res Commun. 1966 Mar 8;22(5):591-6 PMID: 5296448
Transformation of streptococci in chemically defined media.
Biochem Biophys Res Commun. 1967 Jan 23;26(2):181-6 PMID: 6030264
Defined nongrowth media for stage II development of competence in Haemophilus influenzae.
J Bacteriol. 1970 Feb;101(2):517-24 PMID: 5308771
Thymine and thymidine uptake by Haemophilus influenzae and the labeling of deoxyribonucleic acid.
J Bacteriol. 1970 Feb;101(2):525-30 PMID: 5308772
Studies on transformations of Hemophilus influenzae. I. Competence.
J Gen Physiol. 1961 Jul;44:1201-27 PMID: 13707010
A defined medium for Haemophilus influenzae and Haemophilus parainfluenzae.
J Gen Microbiol. 1962 Jan;27:51-60 PMID: 13875197
The growth-factor requirements of Haemopiius influenzae.
J Gen Microbiol. 1962 Feb;27:317-22 PMID: 13908573
Defined medium for Haemophilus influenzae type B.
J Bacteriol. 1963 Jan;85:253-4 PMID: 14001645
A NEW SYNTHETIC MEDIUM FOR DIPLOCOCCUS PNEUMONIAE, AND ITS USE FOR THE STUDY OF RECIPROCAL TRANSFORMATIONS AT THE AMIA LOCUS.
Genetics. 1964 Jul;50:31-44 PMID: 14191356
Defined medium for growth of two transformable strains of Diplococcus pneumoniae.
J Bacteriol. 1959 Aug;78:203-5 PMID: 14436290

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.101.2.513-516.1970

Published

1970-02-00

Pages

513-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC284935

Subset

External Links

PubMed source DOI: 10.1128/jb.101.2.513-516.1970 PMC Full Text View journal details

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