Home LiteratureArticle Details
PMID: 16431929 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

KIF14 and citron kinase act together to promote efficient cytokinesis.

The Journal of cell biology ·Vol. 172 ·No. 3 ·2006-01-30 ·Pages 363-72

Gruneberg U, Neef R, Li X, Chan EH, Chalamalasetty RB, Nigg EA, Barr FA

Abstract

Multiple mitotic kinesins and microtubule-associated proteins (MAPs) act in concert to direct cytokinesis (Glotzer, M. 2005. Science. 307:1735-1739). In anaphase cells, many of these proteins associate with an antiparallel array of microtubules termed the central spindle. The MAP and microtubule-bundling protein PRC1 (protein-regulating cytokinesis 1) is one of the key molecules required for the integrity of this structure (Jiang, W., G. Jimenez, N.J. Wells, T.J. Hope, G.M. Wahl, T. Hunter, and R. Fukunaga. 1998. Mol. Cell. 2:877-885; Mollinari, C., J.P. Kleman, W. Jiang, G. Schoehn, T. Hunter, and R.L. Margolis. 2002. J. Cell Biol. 157:1175-1186). In this study, we identify an interaction between endogenous PRC1 and the previously uncharacterized kinesin KIF14 as well as other mitotic kinesins (MKlp1/CHO1, MKlp2, and KIF4) with known functions in cytokinesis (Hill, E., M. Clarke, and F.A. Barr. 2000. EMBO J. 19:5711-5719; Matuliene, J., and R. Kuriyama. 2002. Mol. Biol. Cell. 13:1832-1845; Kurasawa, Y., W.C. Earnshaw, Y. Mochizuki, N. Dohmae, and K. Todokoro. 2004. EMBO J. 23:3237-3248). We find that KIF14 targets to the central spindle via its interaction with PRC1 and has an essential function in cytokinesis. In KIF14-depleted cells, citron kinase but not other components of the central spindle and cleavage furrow fail to localize. Furthermore, the localization of KIF14 and citron kinase to the central spindle and midbody is codependent, and they form a complex depending on the activation state of citron kinase. Contrary to a previous study (Di Cunto, F., S. Imarisio, E. Hirsch, V. Broccoli, A. Bulfone, A. Migheli, C. Atzori, E. Turco, R. Triolo, G.P. Dotto, et al. 2000. Neuron. 28:115-127), we find a general requirement for citron kinase in human cell division. Together, these findings identify a novel pathway required for efficient cytokinesis.

MeSH Terms
Binding Sites/genetics Cell Cycle Proteins/genetics,metabolism,physiology Cell Line, Tumor Cytokinesis/physiology Cytoplasm/metabolism HeLa Cells Humans Intracellular Signaling Peptides and Proteins Kinesins/genetics,metabolism,physiology Microtubule-Associated Proteins/metabolism Models, Genetic Oncogene Proteins/genetics,metabolism,physiology Protein Binding Protein Serine-Threonine Kinases/genetics,metabolism,physiology RNA, Small Interfering/genetics Spindle Apparatus/metabolism Transfection
Chemicals
Cell Cycle Proteins Intracellular Signaling Peptides and Proteins KIF23 protein, human Microtubule-Associated Proteins Oncogene Proteins PRC1 protein, human RNA, Small Interfering citron-kinase Protein Serine-Threonine Kinases KIF14 protein, human Kinesins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Gruneberg Ulrike
Department of Cell Biology, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany.
Neef Rüdiger
Li Xiuling
Chan Eunice H Y
Chalamalasetty Ravindra B
Nigg Erich A
Barr Francis A
References (40)
40 references, click to expand
  1. Four-dimensional control of the cell cycle.
    Nat Cell Biol. 1999 Jul;1(3):E73-9 PMID: 10559915
  2. pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis.
    Genes Dev. 1998 May 15;12(10):1483-94 PMID: 9585508
  3. Probability-based protein identification by searching sequence databases using mass spectrometry data.
    Electrophoresis. 1999 Dec;20(18):3551-67 PMID: 10612281
  4. Citron, a Rho target that affects contractility during cytokinesis.
    Microsc Res Tech. 2000 Apr 15;49(2):123-6 PMID: 10816250
  5. The Rab6-binding kinesin, Rab6-KIFL, is required for cytokinesis.
    EMBO J. 2000 Nov 1;19(21):5711-9 PMID: 11060022
  6. The FHA domain mediates phosphoprotein interactions.
    J Cell Sci. 2000 Dec;113 Pt 23:4143-9 PMID: 11069759
  7. Defective neurogenesis in citron kinase knockout mice by altered cytokinesis and massive apoptosis.
    Neuron. 2000 Oct;28(1):115-27 PMID: 11086988
  8. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
    Nature. 2001 May 24;411(6836):494-8 PMID: 11373684
  9. Mitotic kinases as regulators of cell division and its checkpoints.
    Nat Rev Mol Cell Biol. 2001 Jan;2(1):21-32 PMID: 11413462
  10. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity.
    Dev Cell. 2002 Jan;2(1):41-54 PMID: 11782313
  11. Kinesin-like protein CHO1 is required for the formation of midbody matrix and the completion of cytokinesis in mammalian cells.
    Mol Biol Cell. 2002 Jun;13(6):1832-45 PMID: 12058052
  12. PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone.
    J Cell Biol. 2002 Jun 24;157(7):1175-86 PMID: 12082078
  13. A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis.
    Dev Cell. 2003 Jan;4(1):29-39 PMID: 12530961
  14. Citron kinase, a Rho-dependent kinase, induces di-phosphorylation of regulatory light chain of myosin II.
    Mol Biol Cell. 2003 May;14(5):1745-56 PMID: 12802051
  15. Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis.
    Mol Biol Cell. 2003 Aug;14(8):3325-41 PMID: 12925766
  16. An intramolecular interaction between the FHA domain and a coiled coil negatively regulates the kinesin motor KIF1A.
    EMBO J. 2004 Apr 7;23(7):1506-15 PMID: 15014437
  17. Role of the midbody matrix in cytokinesis: RNAi and genetic rescue analysis of the mammalian motor protein CHO1.
    Mol Biol Cell. 2004 Jul;15(7):3083-94 PMID: 15075367
  18. Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac.
    J Cell Biol. 2004 Jul 5;166(1):61-71 PMID: 15240570
  19. Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2.
    J Cell Biol. 2004 Jul 19;166(2):167-72 PMID: 15263015
  20. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation.
    EMBO J. 2004 Aug 18;23(16):3237-48 PMID: 15297875
  21. Human chromokinesin KIF4A functions in chromosome condensation and segregation.
    J Cell Biol. 2004 Aug 30;166(5):613-20 PMID: 15326200
  22. Cytokinesis and midzone microtubule organization in Caenorhabditis elegans require the kinesin-like protein ZEN-4.
    Mol Biol Cell. 1998 Aug;9(8):2037-49 PMID: 9693365
  23. Role of citron kinase as a target of the small GTPase Rho in cytokinesis.
    Nature. 1998 Jul 30;394(6692):491-4 PMID: 9697773
  24. A nematode kinesin required for cleavage furrow advancement.
    Curr Biol. 1998 Oct 8;8(20):1133-6 PMID: 9778533
  25. Citron rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein Citron.
    J Biol Chem. 1998 Nov 6;273(45):29706-11 PMID: 9792683
  26. PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis.
    Mol Cell. 1998 Dec;2(6):877-85 PMID: 9885575
  27. Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis.
    J Cell Biol. 2005 Jan 17;168(2):221-32 PMID: 15642749
  28. The molecular requirements for cytokinesis.
    Science. 2005 Mar 18;307(5716):1735-9 PMID: 15774750
  29. Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference.
    Mol Biol Cell. 2005 Jul;16(7):3187-99 PMID: 15843429
  30. Regulation of myosin II during cytokinesis in higher eukaryotes.
    Trends Cell Biol. 2005 Jul;15(7):371-7 PMID: 15935670
  31. KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers.
    Oncogene. 2005 Jul 14;24(30):4741-53 PMID: 15897902
  32. An ECT2-centralspindlin complex regulates the localization and function of RhoA.
    J Cell Biol. 2005 Aug 15;170(4):571-82 PMID: 16103226
  33. Analysis of the kinesin superfamily: insights into structure and function.
    Trends Cell Biol. 2005 Sep;15(9):467-76 PMID: 16084724
  34. Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis.
    J Cell Biol. 2003 Sep 1;162(5):863-75 PMID: 12939256
  35. Terminal cytokinesis events uncovered after an RNAi screen.
    Curr Biol. 2004 Sep 21;14(18):1685-93 PMID: 15380073
  36. Citron kinase is an essential effector of the Pbl-activated Rho signalling pathway in Drosophila melanogaster.
    Development. 2004 Oct;131(20):5053-63 PMID: 15459099
  37. A standardized kinesin nomenclature.
    J Cell Biol. 2004 Oct 11;167(1):19-22 PMID: 15479732
  38. Drosophila citron kinase is required for the final steps of cytokinesis.
    Mol Biol Cell. 2004 Nov;15(11):5053-63 PMID: 15371536
  39. Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis.
    J Cell Sci. 1997 Apr;110 ( Pt 8):945-54 PMID: 9152020
  40. Human ECT2 is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis.
    J Cell Biol. 1999 Nov 29;147(5):921-8 PMID: 10579713
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200511061
Published
2006-01-30
Epub
2006-00-23
Pages
363-72
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2063646
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200511061 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2