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PMID: 16391000 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis.

The Journal of cell biology ·Vol. 172 ·No. 1 ·2006-01-02 ·Pages 103-13

Kuang S, Chargé SB, Seale P, Huh M, Rudnicki MA

Abstract

We assessed viable Pax7(-/-) mice in 129Sv/J background and observed reduced growth and marked muscle wasting together with a complete absence of functional satellite cells. Acute injury resulted in an extreme deficit in muscle regeneration. However, a small number of regenerated myofibers were detected, suggesting the presence of residual myogenic cells in Pax7-deficient muscle. Rare Pax3(+)/MyoD+ myoblasts were recovered from Pax7(-/-) muscle homogenates and cultures of myofiber bundles but not from single myofibers free of interstitial tissues. Finally, we identified Pax3+ cells in the muscle interstitial environment and demonstrated that they coexpressed MyoD during regeneration. Sublaminar satellite cells in hind limb muscle did not express detectable levels of Pax3 protein or messenger RNA. Therefore, we conclude that interstitial Pax3+ cells represent a novel myogenic population that is distinct from the sublaminar satellite cell lineage and that Pax7 is essential for the formation of functional myogenic progenitors from sublaminar satellite cells.

MeSH Terms
Animals Cells, Cultured Mice Mice, Knockout Muscle Development/physiology Muscle, Skeletal/cytology,injuries,physiology MyoD Protein/metabolism PAX3 Transcription Factor PAX7 Transcription Factor/genetics,physiology Paired Box Transcription Factors/physiology Regeneration/physiology Satellite Cells, Skeletal Muscle/cytology
Chemicals
MyoD Protein PAX3 Transcription Factor PAX7 Transcription Factor Paired Box Transcription Factors Pax7 protein, mouse Pax3 protein, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kuang Shihuan
Molecular Medicine Program, Ottawa Health Research Institute, Ottawa, Ontario, Canada.
Chargé Sophie B
Seale Patrick
Huh Michael
Rudnicki Michael A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200508001
Published
2006-01-02
Pages
103-13
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2063538
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200508001 PMC Full Text View journal details
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