Home LiteratureArticle Details

PMID: 16141372 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Direct isolation of satellite cells for skeletal muscle regeneration.

Science (New York, N.Y.) ·Vol. 309 ·No. 5743 ·2005-09-23 ·Pages 2064-7

Montarras D, Morgan J, Collins C, Relaix F, Zaffran S, Cumano A, Partridge T, Buckingham M

Abstract

Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1- cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.

MeSH Terms

Animals Antigens, CD34/analysis Cell Separation Cells, Cultured DNA-Binding Proteins/analysis,biosynthesis Diaphragm Dystrophin/biosynthesis Flow Cytometry Green Fluorescent Proteins/analysis Homeodomain Proteins/analysis,biosynthesis Mice Mice, Inbred mdx Mice, Nude Muscle Development Muscle Fibers, Skeletal/metabolism Muscle, Skeletal/cytology,physiology PAX3 Transcription Factor PAX7 Transcription Factor Paired Box Transcription Factors Regeneration Satellite Cells, Skeletal Muscle/cytology,physiology,transplantation Stem Cell Transplantation Transcription Factors/analysis,biosynthesis

Chemicals

Antigens, CD34 DNA-Binding Proteins Dystrophin Homeodomain Proteins PAX3 Transcription Factor PAX7 Transcription Factor Paired Box Transcription Factors Pax7 protein, mouse Transcription Factors Pax3 protein, mouse Green Fluorescent Proteins

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Montarras Didier

CNRS Unité de Recherche Associée 2578, Department of Developmental Biology, INSERM, Pasteur Institute, 75724 Paris Cedex 15, France. dmontarr@pasteur.fr

Morgan Jennifer

Collins Charlotte

Relaix Frédéric

Zaffran Stéphane

Cumano Ana

Partridge Terence

Buckingham Margaret

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

1095-9203

DOI

10.1126/science.1114758

dmontarr@pasteur.fr

Published

2005-09-23

Epub

2005-00-01

Pages

2064-7

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.1114758 View journal details

Analysis Services

Direct isolation of satellite cells for skeletal muscle regeneration.

Contact

WeChat Official Account