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PMID: 16380438 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells.

The Journal of cell biology ·Vol. 172 ·No. 1 ·2006-01-02 ·Pages 91-102

Relaix F, Montarras D, Zaffran S, Gayraud-Morel B, Rocancourt D, Tajbakhsh S, Mansouri A, Cumano A, Buckingham M

Abstract

The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 and -7 repress MyoD, but do not interfere with the expression of the other myogenic determination factor, Myf5, which, together with Pax3/7, regulates the myogenic differentiation of these cells. In Pax7 mutants, satellite cells are progressively lost in both Pax3-expressing and -nonexpressing muscles. We show that this is caused by satellite cell death, with effects on the cell cycle. Manipulation of the dominant-negative forms of these factors in satellite cell cultures demonstrates that Pax3 cannot replace the antiapoptotic function of Pax7. These findings underline the importance of cell survival in controlling the stem cell populations of adult tissues and demonstrate a role for upstream factors in this context.

MeSH Terms
Animals Apoptosis Cell Cycle Cell Differentiation/genetics Cell Survival/physiology Cells, Cultured Gene Expression Regulation, Developmental/physiology Mice Muscle, Skeletal/cytology Mutation MyoD Protein/metabolism PAX3 Transcription Factor PAX7 Transcription Factor/genetics,physiology Paired Box Transcription Factors/genetics,physiology Satellite Cells, Skeletal Muscle/cytology,physiology
Chemicals
MyoD Protein PAX3 Transcription Factor PAX7 Transcription Factor Paired Box Transcription Factors Pax7 protein, mouse Pax3 protein, mouse
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Relaix Frédéric
Unité de Génétique Moléculaire du Développement, Centre National de la Recherche Scientifique URA 2578, Département de Biologie du Développement.
Montarras Didier
Zaffran Stéphane
Gayraud-Morel Barbara
Rocancourt Didier
Tajbakhsh Shahragim
Mansouri Ahmed
Cumano Ana
Buckingham Margaret
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200508044
Published
2006-01-02
Epub
2005-00-27
Pages
91-102
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2063537
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1083/jcb.200508044 PMC Full Text View journal details
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