Home LiteratureArticle Details
PMID: 16357323 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

T-bet concomitantly controls migration, survival, and effector functions during the development of Valpha14i NKT cells.

Blood ·Vol. 107 ·No. 7 ·2006-04-01 ·Pages 2797-805

Matsuda JL, Zhang Q, Ndonye R, Richardson SK, Howell AR, Gapin L

Abstract

Valpha14i natural killer T (NKT)-cell function has been implicated in a number of disease conditions. The molecular events that drive Valpha14i NKT-cell development remain elusive. We recently showed that T-bet is required for the terminal maturation of these cells. Here we identify some of the genetic targets of T-bet during Valpha14i NKT-cell lineage development. Microarray gene-expression analyses on developing Valpha14i NKT cells were performed and provide a molecular framework to study these maturation events. In vitro ectopic expression of T-bet in immature Valpha14i NKT cells, which do not yet express T-bet, was sufficient to promote Valpha14i NKT-cell maturation, driving the expression of multiple genes, including those that participate in migration, survival, and effector functions. By regulating the expression of T-helper 1 (Th1)-associated cytokines, chemokines, chemokine receptors, and molecules involved in cytolysis, T-bet defines the unique lineage attributes of mature Valpha14i NKT cells and acts to link these attributes to a developmental process.

MeSH Terms
Animals Cell Movement/immunology Cell Survival/immunology Gene Expression Profiling Killer Cells, Natural/cytology,immunology Mice Mice, Inbred C57BL Mice, Knockout Polymerase Chain Reaction Receptors, Antigen, T-Cell/immunology Retroviridae/genetics,immunology T-Box Domain Proteins Transcription Factors/immunology
Chemicals
Receptors, Antigen, T-Cell T-Box Domain Proteins T-box transcription factor TBX21 Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Matsuda Jennifer L
Integrated Department of Immunology, National Jewish Medical and Research Center, University of Colorado Health Science Center, Denver, CO, USA.
Zhang Qianjun
Ndonye Rachel
Richardson Stewart K
Howell Amy R
Gapin Laurent
References (53)
53 references, click to expand
  1. A novel transcription factor, T-bet, directs Th1 lineage commitment.
    Cell. 2000 Mar 17;100(6):655-69 PMID: 10761931
  2. IL-15 induces the expression of chemokines and their receptors in T lymphocytes.
    J Immunol. 1999 Mar 1;162(5):2606-12 PMID: 10072502
  3. Cutting edge: differential expression of chemokines in Th1 and Th2 cells is dependent on Stat6 but not Stat4.
    J Immunol. 2000 Jul 1;165(1):10-4 PMID: 10861028
  4. Tracking the response of natural killer T cells to a glycolipid antigen using CD1d tetramers.
    J Exp Med. 2000 Sep 4;192(5):741-54 PMID: 10974039
  5. Recent developments in the transcriptional regulation of cytolytic effector cells.
    Nat Rev Immunol. 2004 Nov;4(11):900-11 PMID: 15516969
  6. Development of CD1d-restricted NKT cells in the mouse thymus.
    Eur J Immunol. 2004 Dec;34(12):3542-52 PMID: 15549774
  7. T-bet antagonizes mSin3a recruitment and transactivates a fully methylated IFN-gamma promoter via a conserved T-box half-site.
    Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):2034-9 PMID: 15684083
  8. Developmental program of mouse Valpha14i NKT cells.
    Curr Opin Immunol. 2005 Apr;17(2):122-30 PMID: 15766670
  9. Toward an understanding of NKT cell biology: progress and paradoxes.
    Annu Rev Immunol. 2005;23:877-900 PMID: 15771592
  10. Expansion and long-range differentiation of the NKT cell lineage in mice expressing CD1d exclusively on cortical thymocytes.
    J Exp Med. 2005 Jul 18;202(2):239-48 PMID: 16027237
  11. The influence of CD1d in postselection NKT cell maturation and homeostasis.
    J Immunol. 2005 Sep 15;175(6):3762-8 PMID: 16148122
  12. Commitment toward the natural T (iNKT) cell lineage occurs at the CD4+8+ stage of thymic ontogeny.
    Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5114-9 PMID: 15792999
  13. Intravascular immune surveillance by CXCR6+ NKT cells patrolling liver sinusoids.
    PLoS Biol. 2005 Apr;3(4):e113 PMID: 15799695
  14. Genetic evidence supporting selection of the Valpha14i NKT cell lineage from double-positive thymocyte precursors.
    Immunity. 2005 Jun;22(6):705-16 PMID: 15963785
  15. The primate lentiviral receptor Bonzo/STRL33 is coordinately regulated with CCR5 and its expression pattern is conserved between human and mouse.
    J Immunol. 2000 Sep 15;165(6):3284-92 PMID: 10975845
  16. TRAIL expression by activated human CD4(+)V alpha 24NKT cells induces in vitro and in vivo apoptosis of human acute myeloid leukemia cells.
    Blood. 2001 Apr 1;97(7):2067-74 PMID: 11264173
  17. Early transcription and silencing of cytokine genes underlie polarization of T helper cell subsets.
    Immunity. 2001 Mar;14(3):205-15 PMID: 11290331
  18. NKT cells derive from double-positive thymocytes that are positively selected by CD1d.
    Nat Immunol. 2001 Oct;2(10):971-8 PMID: 11550008
  19. Final steps of natural killer cell maturation: a model for type 1-type 2 differentiation?
    Nat Immunol. 2001 Oct;2(10):917-24 PMID: 11577347
  20. Natural killer T cells reactive to a single glycolipid exhibit a highly diverse T cell receptor beta repertoire and small clone size.
    Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12636-41 PMID: 11592984
  21. Natural killer T cells restricted by the monomorphic MHC class 1b CD1d1 molecules behave like inflammatory cells.
    J Immunol. 2002 Jan 1;168(1):365-71 PMID: 11751982
  22. Distinct effects of T-bet in TH1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells.
    Science. 2002 Jan 11;295(5553):338-42 PMID: 11786644
  23. A natural killer T (NKT) cell developmental pathway iInvolving a thymus-dependent NK1.1(-)CD4(+) CD1d-dependent precursor stage.
    J Exp Med. 2002 Apr 1;195(7):835-44 PMID: 11927628
  24. A thymic precursor to the NK T cell lineage.
    Science. 2002 Apr 19;296(5567):553-5 PMID: 11968185
  25. In vivo developmental stages in murine natural killer cell maturation.
    Nat Immunol. 2002 Jun;3(6):523-8 PMID: 12006976
  26. NKT cell-derived RANTES recruits APCs and CD8+ T cells to the spleen during the generation of regulatory T cells in tolerance.
    J Immunol. 2002 Jul 1;169(1):31-8 PMID: 12077225
  27. Hlx is induced by and genetically interacts with T-bet to promote heritable T(H)1 gene induction.
    Nat Immunol. 2002 Jul;3(7):652-8 PMID: 12055627
  28. The unconventional lifestyle of NKT cells.
    Nat Rev Immunol. 2002 Aug;2(8):557-68 PMID: 12154375
  29. NK T cell precursors exhibit differential cytokine regulation and require Itk for efficient maturation.
    J Immunol. 2002 Sep 1;169(5):2397-406 PMID: 12193707
  30. Homeostasis of V alpha 14i NKT cells.
    Nat Immunol. 2002 Oct;3(10):966-74 PMID: 12244311
  31. Molecular and functional profiling of memory CD8 T cell differentiation.
    Cell. 2002 Dec 13;111(6):837-51 PMID: 12526810
  32. IL-15 availability conditions homeostasis of peripheral natural killer T cells.
    Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2663-8 PMID: 12598649
  33. CD1d-restricted NKT cells express a chemokine receptor profile indicative of Th1-type inflammatory homing cells.
    J Immunol. 2003 Sep 1;171(5):2571-80 PMID: 12928408
  34. Differential chemokine responses and homing patterns of murine TCR alpha beta NKT cell subsets.
    J Immunol. 2003 Sep 15;171(6):2960-9 PMID: 12960320
  35. Constitutive cytokine mRNAs mark natural killer (NK) and NK T cells poised for rapid effector function.
    J Exp Med. 2003 Oct 6;198(7):1069-76 PMID: 14530376
  36. Control of effector CD8+ T cell function by the transcription factor Eomesodermin.
    Science. 2003 Nov 7;302(5647):1041-3 PMID: 14605368
  37. Differential roles for IL-15R alpha-chain in NK cell development and Ly-49 induction.
    J Immunol. 2003 Nov 15;171(10):5085-90 PMID: 14607906
  38. The role of activation-induced cell death in the differentiation of T-helper-cell subsets.
    Immunol Res. 2003;28(3):285-93 PMID: 14713720
  39. The VITAL assay: a versatile fluorometric technique for assessing CTL- and NKT-mediated cytotoxicity against multiple targets in vitro and in vivo.
    J Immunol Methods. 2004 Feb 1;285(1):25-40 PMID: 14871532
  40. Kinetic analysis of genomewide gene expression reveals molecule circuitries that control T cell activation and Th1/2 differentiation.
    Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3023-8 PMID: 14978277
  41. The dynamic life of natural killer cells.
    Annu Rev Immunol. 2004;22:405-29 PMID: 15032583
  42. CD1: antigen presentation and T cell function.
    Annu Rev Immunol. 2004;22:817-90 PMID: 15032598
  43. NKT cells: what's in a name?
    Nat Rev Immunol. 2004 Mar;4(3):231-7 PMID: 15039760
  44. T-bet regulates the terminal maturation and homeostasis of NK and Valpha14i NKT cells.
    Immunity. 2004 Apr;20(4):477-94 PMID: 15084276
  45. Sustained T-bet expression confers polarized human TH2 cells with TH1-like cytokine production and migratory capacities.
    J Allergy Clin Immunol. 2004 May;113(5):987-94 PMID: 15131585
  46. Cutting Edge: Innate production of IFN-gamma by NK cells is independent of epigenetic modification of the IFN-gamma promoter.
    J Immunol. 2004 Aug 1;173(3):1514-7 PMID: 15265878
  47. Evolutionarily conserved sequence elements that positively regulate IFN-gamma expression in T cells.
    Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12622-7 PMID: 15304658
  48. Cytotoxicity of fresh NK1.1+ T cell receptor alpha/beta+ thymocytes against a CD4+8+ thymocyte population associated with intact Fas antigen expression on the target.
    J Exp Med. 1994 Aug 1;180(2):423-32 PMID: 7519236
  49. An invariant T cell receptor alpha chain is used by a unique subset of major histocompatibility complex class I-specific CD4+ and CD4-8- T cells in mice and humans.
    J Exp Med. 1994 Sep 1;180(3):1097-106 PMID: 7520467
  50. Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s.
    J Exp Med. 1998 Jan 5;187(1):129-34 PMID: 9419219
  51. The T-box gene family.
    Bioessays. 1998 Jan;20(1):9-19 PMID: 9504043
  52. Natural killer-like nonspecific tumor cell lysis mediated by specific ligand-activated Valpha14 NKT cells.
    Proc Natl Acad Sci U S A. 1998 May 12;95(10):5690-3 PMID: 9576945
  53. A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM).
    J Leukoc Biol. 2000 May;67(5):725-34 PMID: 10811014
Article Info
Journal
Blood
Abbr.
Blood
ISSN
0006-4971
DOI
10.1182/blood-2005-08-3103
Published
2006-04-01
Epub
2005-00-15
Pages
2797-805
Language
English
Region
United States
NLM ID
7603509
PMCID
PMC1895373
Subset
IM
Grants
NIAID NIH HHS · AI057485 · United States
NIAID NIH HHS · AI057519 · United States
NIAID NIH HHS · T32AI00048 · United States
External Links
PubMed source DOI: 10.1182/blood-2005-08-3103 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2