Home LiteratureArticle Details
PMID: 16306146 Published · ppublish English Journal Article

Pathogen-responsive expression of glycosyltransferase genes UGT73B3 and UGT73B5 is necessary for resistance to Pseudomonas syringae pv tomato in Arabidopsis.

Plant physiology ·Vol. 139 ·No. 4 ·2005-12-00 ·Pages 1890-901

Langlois-Meurinne M, Gachon CM, Saindrenan P

Abstract

The genome sequencing of Arabidopsis (Arabidopsis thaliana) has revealed that secondary metabolism plant glycosyltransferases (UGTs) are encoded by an unexpectedly large multigenic family of 120 members. Very little is known about their actual function in planta, in particular during plant pathogen interactions. Among them, members of the group D are of particular interest since they are related to UGTs involved in stress-inducible responses in other plant species. We provide here a detailed analysis of the expression profiles of this group of Arabidopsis UGTs following infection with Pseudomonas syringae pv tomato or after treatment with salicylic acid, methyljasmonate, and hydrogen peroxide. Members of the group D displayed distinct induction profiles, indicating potential roles in stress or defense responses notably for UGT73B3 and UGT73B5. Analysis of UGT expression in Arabidopsis defense-signaling mutants further revealed that their induction is methyljasmonate independent, but partially salicylic acid dependent. T-DNA tagged mutants (ugt73b3 and ugt73b5) exhibited decreased resistance to P. syringae pv tomato-AvrRpm1, indicating that expression of the corresponding UGT genes is necessary during the hypersensitive response. These results emphasize the importance of plant secondary metabolite UGTs in plant-pathogen interactions and provide foundation for future understanding of the exact role of UGTs during the hypersensitive response.

MeSH Terms
Acetates/pharmacology Arabidopsis/enzymology,genetics,microbiology Arabidopsis Proteins/classification,genetics,metabolism Base Sequence Cyclopentanes/pharmacology DNA, Plant/genetics Gene Expression Genes, Plant Glucosyltransferases/classification,genetics,metabolism Glycosyltransferases/classification,genetics,metabolism Hydrogen Peroxide/pharmacology Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Oxylipins Phylogeny Pseudomonas syringae/pathogenicity Salicylic Acid/metabolism,pharmacology Signal Transduction Virulence
Chemicals
Acetates Arabidopsis Proteins Cyclopentanes DNA, Plant Oxylipins methyl jasmonate Hydrogen Peroxide Glycosyltransferases Glucosyltransferases UGT73B3 protein, Arabidopsis UGT73B5 protein, Arabidopsis Salicylic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Langlois-Meurinne Mathilde
Institut de Biotechnologie des Plantes, Centre National de la Recherche Scientifique-Université Paris-Sud, Unité Mixte de Recherche 8618, 91405 Orsay cedex, France.
Gachon Claire M M
Saindrenan Patrick
References (64)
64 references, click to expand
  1. Development and evaluation of an Arabidopsis whole genome Affymetrix probe array.
    Plant J. 2004 May;38(3):545-61 PMID: 15086809
  2. Genomics-based selection and functional characterization of triterpene glycosyltransferases from the model legume Medicago truncatula.
    Plant J. 2005 Mar;41(6):875-87 PMID: 15743451
  3. Plant glutathione S-transferases: enzymes with multiple functions in sickness and in health.
    Trends Plant Sci. 2000 May;5(5):193-8 PMID: 10785664
  4. The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates.
    J Biol Chem. 2002 Jan 4;277(1):586-92 PMID: 11641410
  5. Plant secondary metabolism glycosyltransferases: the emerging functional analysis.
    Trends Plant Sci. 2005 Nov;10(11):542-9 PMID: 16214386
  6. Genome-wide insertional mutagenesis of Arabidopsis thaliana.
    Science. 2003 Aug 1;301(5633):653-7 PMID: 12893945
  7. Over-expression of a scopoletin glucosyltransferase in Nicotiana tabacum leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance.
    Plant Mol Biol. 2004 Jan;54(1):137-46 PMID: 15159640
  8. Fumonisin B1-induced cell death in arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways.
    Plant Cell. 2000 Oct;12(10):1823-36 PMID: 11041879
  9. Semi-constitutive expression of an Arabidopsis thaliana alpha-tubulin gene.
    Plant Mol Biol. 1993 Mar;21(5):937-42 PMID: 8467087
  10. Genetic control of UDPglucose:flavonol 3-O-glucosyltransferase in the endosperm of maize.
    Biochem Genet. 1977 Jun;15(5-6):509-19 PMID: 880210
  11. UGT73C6 and UGT78D1, glycosyltransferases involved in flavonol glycoside biosynthesis in Arabidopsis thaliana.
    J Biol Chem. 2003 Nov 7;278(45):43910-8 PMID: 12900416
  12. Downregulation of a pathogen-responsive tobacco UDP-Glc:phenylpropanoid glucosyltransferase reduces scopoletin glucoside accumulation, enhances oxidative stress, and weakens virus resistance.
    Plant Cell. 2002 May;14(5):1093-107 PMID: 12034899
  13. Arabidopsis Flavonoid Mutants Are Hypersensitive to UV-B Irradiation.
    Plant Cell. 1993 Feb;5(2):171-179 PMID: 12271060
  14. Cloning and expression of solanidine UDP-glucose glucosyltransferase from potato.
    Plant J. 1997 Feb;11(2):227-36 PMID: 9076990
  15. Glycosyltransferases in plant natural product synthesis: characterization of a supergene family.
    Trends Plant Sci. 2000 Sep;5(9):380-6 PMID: 10973093
  16. Loss of a callose synthase results in salicylic acid-dependent disease resistance.
    Science. 2003 Aug 15;301(5635):969-72 PMID: 12920300
  17. Two tobacco genes induced by infection, elicitor and salicylic acid encode glucosyltransferases acting on phenylpropanoids and benzoic acid derivatives, including salicylic acid.
    FEBS Lett. 1998 Oct 23;437(3):319-23 PMID: 9824316
  18. Molecular cloning and characterization of a glucosyltransferase catalyzing glucosylation of curcumin in cultured Catharanthus roseus cells.
    FEBS Lett. 2004 Jun 4;567(2-3):197-202 PMID: 15178322
  19. The rapid induction of glutathione S-transferases AtGSTF2 and AtGSTF6 by avirulent Pseudomonas syringae is the result of combined salicylic acid and ethylene signaling.
    Plant Cell Physiol. 2003 Jul;44(7):750-7 PMID: 12881503
  20. Reactive oxygen species: metabolism, oxidative stress, and signal transduction.
    Annu Rev Plant Biol. 2004;55:373-99 PMID: 15377225
  21. The jasmonate pathway.
    Science. 2002 May 31;296(5573):1649-50 PMID: 12040182
  22. Differential expression and evolution of the Arabidopsis CYP86A subfamily.
    Plant Physiol. 2005 Mar;137(3):1067-81 PMID: 15709153
  23. Resistance gene-dependent plant defense responses.
    Plant Cell. 1996 Oct;8(10):1773-91 PMID: 8914325
  24. Over-expression of an Arabidopsis gene encoding a glucosyltransferase of indole-3-acetic acid: phenotypic characterisation of transgenic lines.
    Plant J. 2002 Nov;32(4):573-83 PMID: 12445128
  25. Cloning and expression of a cDNA encoding betanidin 5-O-glucosyltransferase, a betanidin- and flavonoid-specific enzyme with high homology to inducible glucosyltransferases from the Solanaceae.
    Plant J. 1999 Sep;19(5):509-19 PMID: 10504573
  26. NPR1, all things considered.
    Curr Opin Plant Biol. 2004 Oct;7(5):547-52 PMID: 15337097
  27. GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox.
    Plant Physiol. 2004 Sep;136(1):2621-32 PMID: 15375207
  28. Natural products and plant disease resistance.
    Nature. 2001 Jun 14;411(6839):843-7 PMID: 11459067
  29. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
    Nucleic Acids Res. 1997 Dec 15;25(24):4876-82 PMID: 9396791
  30. Identification of an immediate-early salicylic acid-inducible tobacco gene and characterization of induction by other compounds.
    Plant Mol Biol. 1996 Aug;31(5):1061-72 PMID: 8843948
  31. Higher plant glycosyltransferases.
    Genome Biol. 2001;2(2):REVIEWS3004 PMID: 11182895
  32. Identification and biochemical characterization of an Arabidopsis indole-3-acetic acid glucosyltransferase.
    J Biol Chem. 2001 Feb 9;276(6):4350-6 PMID: 11042207
  33. Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor.
    Plant J. 2005 Apr;42(2):218-35 PMID: 15807784
  34. H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response.
    Cell. 1994 Nov 18;79(4):583-93 PMID: 7954825
  35. A class of plant glycosyltransferases involved in cellular homeostasis.
    EMBO J. 2004 Aug 4;23(15):2915-22 PMID: 15241472
  36. Identification of glucosyltransferase genes involved in sinapate metabolism and lignin synthesis in Arabidopsis.
    J Biol Chem. 2001 Feb 9;276(6):4344-9 PMID: 11042211
  37. Transcriptional similarities, dissimilarities, and conservation of cis-elements in duplicated genes of Arabidopsis.
    Plant Physiol. 2004 Oct;136(2):3009-22 PMID: 15489284
  38. A computer program for choosing optimal oligonucleotides for filter hybridization, sequencing and in vitro amplification of DNA.
    Nucleic Acids Res. 1989 Nov 11;17(21):8543-51 PMID: 2587212
  39. Acquired resistance in Arabidopsis.
    Plant Cell. 1992 Jun;4(6):645-56 PMID: 1392589
  40. Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana.
    J Biol Chem. 2003 Nov 28;278(48):47905-14 PMID: 12970342
  41. Four classes of salicylate-induced tobacco genes.
    Mol Plant Microbe Interact. 1998 Sep;11(9):895-905 PMID: 9725022
  42. Multiple secondary plant product UDP-glucose glucosyltransferase genes expressed in cassava (Manihot esculenta Crantz) cotyledons.
    DNA Seq. 1994;5(1):41-9 PMID: 7894058
  43. Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis.
    Plant J. 2004 Dec;40(6):893-908 PMID: 15584955
  44. Light induces phenylpropanoid metabolism in Arabidopsis roots.
    Plant J. 2004 Jun;38(5):765-78 PMID: 15144378
  45. Glucose conjugation of anthranilate by the Arabidopsis UGT74F2 glucosyltransferase is required for tryptophan mutant blue fluorescence.
    J Biol Chem. 2003 Feb 21;278(8):6275-81 PMID: 12475971
  46. Trends in lignin modification: a comprehensive analysis of the effects of genetic manipulations/mutations on lignification and vascular integrity.
    Phytochemistry. 2002 Oct;61(3):221-94 PMID: 12359514
  47. Contributing software to the internet: the Amplify program.
    Trends Biochem Sci. 1993 Nov;18(11):448-50 PMID: 8291093
  48. Real-time PCR: what relevance to plant studies?
    J Exp Bot. 2004 Jul;55(402):1445-54 PMID: 15208338
  49. Transcriptional co-regulation of secondary metabolism enzymes in Arabidopsis: functional and evolutionary implications.
    Plant Mol Biol. 2005 May;58(2):229-45 PMID: 16027976
  50. Cloning and expression of UDP-glucose: flavonoid 7-O-glucosyltransferase from hairy root cultures of Scutellaria baicalensis.
    Planta. 2000 May;210(6):1006-13 PMID: 10872235
  51. cDNA cloning and expression of isoflavonoid-specific glucosyltransferase from Glycyrrhiza echinata cell-suspension cultures.
    Planta. 2004 Jan;218(3):456-9 PMID: 14523650
  52. Cloning and characterization of the abscisic acid-specific glucosyltransferase gene from adzuki bean seedlings.
    Plant Physiol. 2002 Jul;129(3):1285-95 PMID: 12114582
  53. A central role of salicylic Acid in plant disease resistance.
    Science. 1994 Nov 18;266(5188):1247-50 PMID: 17810266
  54. Characterization of an Arabidopsis Mutant That Is Nonresponsive to Inducers of Systemic Acquired Resistance.
    Plant Cell. 1994 Nov;6(11):1583-1592 PMID: 12244227
  55. Ectopic expression of a UDP-glucose:phenylpropanoid glucosyltransferase leads to increased resistance of transgenic tobacco plants against infection with Potato Virus Y.
    Plant Cell Physiol. 2004 Sep;45(9):1185-93 PMID: 15509841
  56. Expression profiling of the whole Arabidopsis shaggy-like kinase multigene family by real-time reverse transcriptase-polymerase chain reaction.
    Plant Physiol. 2002 Oct;130(2):577-90 PMID: 12376626
  57. Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers.
    Planta. 2001 Jun;213(2):164-74 PMID: 11469580
  58. Early genes and auxin action.
    Plant Physiol. 1996 May;111(1):9-17 PMID: 8685277
  59. On the origin of family 1 plant glycosyltransferases.
    Phytochemistry. 2003 Feb;62(3):399-413 PMID: 12620353
  60. Biochemical and molecular characterization of a novel UDP-glucose:anthocyanin 3'-O-glucosyltransferase, a key enzyme for blue anthocyanin biosynthesis, from gentian.
    Plant Physiol. 2003 Jul;132(3):1652-63 PMID: 12857844
  61. Cloning and regiospecificity studies of two flavonoid glucosyltransferases from Allium cepa.
    Phytochemistry. 2003 Nov;64(6):1069-76 PMID: 14568073
  62. A novel tomato gene that rapidly responds to wound- and pathogen-related signals.
    Plant J. 1998 Apr;14(1):137-42 PMID: 15494059
  63. Molecular phenotyping of the pal1 and pal2 mutants of Arabidopsis thaliana reveals far-reaching consequences on phenylpropanoid, amino acid, and carbohydrate metabolism.
    Plant Cell. 2004 Oct;16(10):2749-71 PMID: 15377757
  64. NPR1-independent activation of immediate early salicylic acid-responsive genes in Arabidopsis.
    Mol Plant Microbe Interact. 2004 Jan;17(1):34-42 PMID: 14714866
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.105.067223
Published
2005-12-00
Epub
2005-00-23
Pages
1890-901
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1310567
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.105.067223 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2