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PMID: 16291644 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Regulation of an intergenic transcript controls adjacent gene transcription in Saccharomyces cerevisiae.

Genes & development ·Vol. 19 ·No. 22 ·2005-11-15 ·Pages 2695-704

Martens JA, Wu PY, Winston F

Abstract

Recent studies have revealed that transcription of noncoding, intergenic DNA is abundant among eukaryotes. However, the functions of this transcription are poorly understood. We have previously shown that in Saccharomyces cerevisiae, expression of an intergenic transcript, SRG1, represses the transcription of the adjacent gene, SER3, by transcription interference. We now show that SRG1 transcription is regulated by serine, thereby conferring regulation of SER3, a serine biosynthetic gene. This regulation requires Cha4, a serine-dependent activator that binds to the SRG1 promoter and is required for SRG1 induction in the presence of serine. Furthermore, two coactivator complexes, SAGA and Swi/Snf, are also directly required for activation of SRG1 and transcription interference of SER3. Taken together, our results elucidate a physiological role for intergenic transcription in the regulation of SER3. Moreover, our results demonstrate a mechanism by which intergenic transcription allows activators to act indirectly as repressors.

MeSH Terms
DNA, Intergenic/genetics DNA-Binding Proteins/metabolism Drosophila Proteins/physiology Gene Expression Regulation, Fungal/physiology Phosphoglycerate Dehydrogenase/antagonists & inhibitors,biosynthesis,genetics Promoter Regions, Genetic RNA-Binding Proteins/physiology Repressor Proteins/physiology Ribonucleoprotein, U1 Small Nuclear/physiology Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/antagonists & inhibitors,biosynthesis,genetics,metabolism,physiology Serine/physiology Suppression, Genetic/genetics Trans-Activators/metabolism Transcription Factors/physiology Transcription, Genetic/physiology
Chemicals
CHA4 protein, S cerevisiae DNA, Intergenic DNA-Binding Proteins Drosophila Proteins RNA-Binding Proteins Repressor Proteins Ribonucleoprotein, U1 Small Nuclear SPT3 protein, S cerevisiae SPT8 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors snf protein, Drosophila Serine Phosphoglycerate Dehydrogenase SER3 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Martens Joseph A
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Wu Pei-Yun Jenny
Winston Fred
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1367605
Published
2005-11-15
Pages
2695-704
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1283962
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032967 · United States
NIGMS NIH HHS · R37 GM032967 · United States
NIGMS NIH HHS · GM32967 · United States
External Links
PubMed source DOI: 10.1101/gad.1367605 PMC Full Text View journal details
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