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PMID: 1628811 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Somatic instability of a Drosophila chromosome.

Genetics ·Vol. 131 ·No. 3 ·1992-07-00 ·Pages 683-91

Wines DR, Henikoff S

Abstract

A mitotically unstable chromosome, detectable because of mosaic expression of marker genes, was generated by X-ray mutagenesis in Drosophila. Nondisjunction of this chromosome is evident in mitotic chromosome preparations, and premature sister chromatid separation is frequent. The mosaic phenotype is modified by genetic elements that are thought to alter chromatin structure. We hypothesize that the mitotic defects result from a breakpoint deep in the pericentric heterochromatin, within or very near to the DNA sequences essential for centromere function. This unique chromosome may provide a tool for the genetic and molecular dissection of a higher eukaryotic centromere.

Related Genes
LacZ
MeSH Terms
Animals Centromere Chromosomes/radiation effects Crosses, Genetic Drosophila/genetics Female Genes, Dominant Genetic Markers Immunohistochemistry Karyotyping Male Mitosis Multigene Family Mutagenesis Nondisjunction, Genetic Nucleic Acid Hybridization Translocation, Genetic beta-Galactosidase/metabolism
Chemicals
Genetic Markers beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wines D R
Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Henikoff S
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/131.3.683
Published
1992-07-00
Pages
683-91
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205039
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/131.3.683 PMC Full Text View journal details
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