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PMID: 1628629 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An endonuclease with multiple cutting sites, Endo.SceI, initiates genetic recombination at its cutting site in yeast mitochondria.

The EMBO journal ·Vol. 11 ·No. 7 ·1992-07-00 ·Pages 2707-15

Nakagawa K, Morishima N, Shibata T

Abstract

Endo.SceI is a mitochondrial sequence-specific endonuclease which has multiple cutting sites. In order to examine the possible role of Endo.SceI in homologous recombination, we analyzed the mode of recombination upon mating using antibiotic resistance markers on the mitochondrial genome. The segregation of a marker located very close to one of the Endo.SceI cutting sites showed a disparity (polarized segregation, i.e. gene conversion). This gene conversion depended on the presence of the functional Endo.SceI gene. In vivo cutting of mitochondrial DNA upon mating was detected at the cutting site in the antibiotic marker region, which also depended on the Endo.SceI activity. These results suggest that mitochondrial recombination is induced by cleavage of mitochondrial DNA by this sequence-specific endonuclease. This is the first demonstration that a sequence-specific endonuclease with multiple cutting sites induces genetic recombination.

Related Genes
ENS2 Endo.SceI oli2
MeSH Terms
Base Sequence Blotting, Southern DNA, Fungal/metabolism DNA, Mitochondrial/metabolism Deoxyribonucleases, Type II Site-Specific/genetics,metabolism Drug Resistance, Microbial/genetics Gene Conversion Genes, Fungal Molecular Sequence Data Recombination, Genetic Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
DNA, Fungal DNA, Mitochondrial Saccharomyces cerevisiae Proteins SCEI protein, S cerevisiae Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nakagawa K
Laboratory of Microbiology, RIKEN Institute of Physical and Chemical Research, Saitama, Japan.
Morishima N
Shibata T
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44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-07-00
Pages
2707-15
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556746
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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