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PMID: 16275750 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Centrobin: a novel daughter centriole-associated protein that is required for centriole duplication.

The Journal of cell biology ·Vol. 171 ·No. 3 ·2005-11-07 ·Pages 437-45

Zou C, Li J, Bai Y, Gunning WT, Wazer DE, Band V, Gao Q

Abstract

In mammalian cells, the centrosome consists of a pair of centrioles and amorphous pericentriolar material. The pair of centrioles, which are the core components of the centrosome, duplicate once per cell cycle. Centrosomes play a pivotal role in orchestrating the formation of the bipolar spindle during mitosis. Recent studies have linked centrosomal activity on centrioles or centriole-associated structures to cytokinesis and cell cycle progression through G1 into the S phase. In this study, we have identified centrobin as a centriole-associated protein that asymmetrically localizes to the daughter centriole. The silencing of centrobin expression by small interfering RNA inhibited centriole duplication and resulted in centrosomes with one or no centriole, demonstrating that centrobin is required for centriole duplication. Furthermore, inhibition of centriole duplication by centrobin depletion led to impaired cytokinesis.

MeSH Terms
Amino Acid Sequence Animals Cell Cycle Proteins/genetics,physiology Cell Line Centrioles/physiology,ultrastructure Centrosome/ultrastructure Cytokinesis Gene Silencing Humans Mice Microscopy, Immunoelectron Molecular Sequence Data RNA, Small Interfering/genetics
Chemicals
CNTROB protein, human Cell Cycle Proteins RNA, Small Interfering
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zou Chaozhong
Department of Medicine, Division of Cancer Biology, Evanston Northwestern Healthcare Research Institute, Northwestern University Feinberg School of Medicine, Evanston, IL 60201, USA.
Li Jun
Bai Yujie
Gunning William T
Wazer David E
Band Vimla
Gao Qingshen
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200506185
Published
2005-11-07
Pages
437-45
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171251
Subset
IM
Grants
NCI NIH HHS · R01 CA095221 · United States
NCI NIH HHS · R01 CA096986 · United States
NCI NIH HHS · 1 R01 CA095221-01A1 · United States
NCI NIH HHS · 1 R01 CA96986-01A1 · United States
External Links
PubMed source DOI: 10.1083/jcb.200506185 PMC Full Text View journal details
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