Insights into the stress response and sulfur metabolism revealed by proteome analysis of a Chlorobium tepidum mutant lacking the Rubisco-like protein.

Photosynthesis research ·Vol. 78 ·No. 3 ·2003-00-00 ·Pages 231-48

Hanson TE, Tabita FR

Abstract

A significant fraction of the proteome of Chlorobium tepidum is altered in a mutant strain of the green sulfur bacterium C. tepidum (Omega::RLP) lacking the Rubisco-like protein (RLP). Additionally, a number of stress proteins display altered abundance or migration in strain Omega::RLP, including a thioredoxin, a putative Hsp20 family chaperonin, and GroEL. Changes in protein abundance are closely correlated to mRNA abundance in the case of two other stress proteins, a thiol-specific antioxidant protein homolog (Tsa/AhpC) and an iron only superoxide dismutase (Fe-SOD). Strain Omega::RLP is more resistant to hydrogen peroxide exposure than strain WT2321, providing evidence that the stress proteins are functional. Strain Omega::RLP is also defective in thiosulfate oxidation, but is able to oxidize sulfide as well as the wild-type strain. Based on studies with periplasm-enriched extracts of strain Omega::RLP, the loss of thiosulfate oxidation capability correlates with undetectable levels of the Sox Y protein, a component of the predicted thiosulfate oxidation complex. These results provide further indications that sulfur oxidation capacity and the response to stress are linked in C. tepidum, with the RLP playing a major role.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hanson Thomas E

Graduate College of Marine Studies and Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711, USA.

Tabita F Robert

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Article Info

Journal

Photosynthesis research

Abbr.

Photosynth Res

ISSN

1573-5079

DOI

10.1023/B:PRES.0000006829.41444.3d

Published

2003-00-00

Pages

231-48

Language

English

Region

Netherlands

NLM ID

100954728

External Links

PubMed source DOI: 10.1023/B:PRES.0000006829.41444.3d View journal details

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Insights into the stress response and sulfur metabolism revealed by proteome analysis of a Chlorobium tepidum mutant lacking the Rubisco-like protein.

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