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PMID: 7730296 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Genetic transfer by conjugation in the thermophilic green sulfur bacterium Chlorobium tepidum.

Journal of bacteriology ·Vol. 177 ·No. 9 ·1995-05-00 ·Pages 2583-8

Wahlund TM, Madigan MT

Abstract

The broad-host-range IncQ group plasmids pDSK519 and pGSS33 were transferred by conjugation from Escherichia coli into the thermophilic green sulfur bacterium Chlorobium tepidum. C. tepidum exconjugants expressed the kanamycin and ampicillin-chloramphenicol resistances encoded by pDSK519 and pGSS33, respectively. Ampicillin resistance was a particularly good marker for selection in C. tepidum. Both pDSK519 and pGSS33 were stably maintained in C. tepidum at temperatures below 42 degrees C and could be transferred between C. tepidum and E. coli without modifications. Conjugation frequencies ranged from 10(-1) to 10(-4) exconjugants per donor cell, and frequencies of 10(-2) to 10(-3) were consistently obtained when ampicillin resistance was used as a selectable marker. Methods for growth of C. tepidum on agar, isolation of plating strains and antibiotic-resistant mutants of wild-type C. tepidum cells, and optimum conditions for conjugation were also investigated.

MeSH Terms
Bacteria/genetics,growth & development Conjugation, Genetic Drug Resistance, Microbial/genetics Gene Transfer Techniques Genetic Markers Photosynthesis/genetics Plasmids/genetics Selection, Genetic
Chemicals
Genetic Markers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wahlund T M
Department of Microbiology, Southern Illinois University, Carbondale 62901-6508, USA.
Madigan M T
References (17)
17 references, click to expand
  1. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
  2. Redox effects on the bacteriochlorophyll a-containing Fenna-Matthews-Olson protein from Chlorobium tepidum.
    Photosynth Res. 1994 Jul;41(1):89-96 PMID: 11539857
  3. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  4. Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis.
    J Bacteriol. 1982 Mar;149(3):824-30 PMID: 6277855
  5. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas.
    Gene. 1981 Dec;16(1-3):237-47 PMID: 6282695
  6. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  7. Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1561-5 PMID: 6324204
  8. Broad host range cloning vectors for gram-negative bacteria.
    Gene. 1984 Jul-Aug;29(1-2):93-102 PMID: 6092235
  9. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.
    Gene. 1988 Oct 15;70(1):191-7 PMID: 2853689
  10. A new family of RSF1010-derived expression and lac-fusion broad-host-range vectors for gram-negative bacteria.
    Gene. 1990 Apr 30;89(1):37-46 PMID: 2115488
  11. Photosynthetic reaction center genes in green sulfur bacteria and in photosystem 1 are related.
    Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8135-9 PMID: 1518838
  12. Plasmid transfer by conjugation in Desulfovibrio desulfuricans.
    FEMS Microbiol Lett. 1992 Jul 15;73(3):255-62 PMID: 1426989
  13. Nitrogen fixation by the thermophilic green sulfur bacterium Chlorobium tepidum.
    J Bacteriol. 1993 Jan;175(2):474-8 PMID: 8093448
  14. Biochemical genetics revisited: the use of mutants to study carbon and nitrogen metabolism in the photosynthetic bacteria.
    FEMS Microbiol Rev. 1993 Jan;10(1-2):1-38 PMID: 8431308
  15. The atp2 operon of the green bacterium Chlorobium limicola.
    Biochim Biophys Acta. 1993 Mar 20;1172(3):267-73 PMID: 8448205
  16. Transformation of Chlorobium limicola by a plasmid that confers the ability to utilize thiosulfate.
    J Bacteriol. 1994 Dec;176(23):7395-7 PMID: 7893283
  17. Plasmids of Escherichia coli as cloning vectors.
    Methods Enzymol. 1979;68:245-67 PMID: 232214
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.9.2583-2588.1995
Published
1995-05-00
Pages
2583-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC176923
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.177.9.2583-2588.1995 PMC Full Text View journal details
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