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PMID: 16172097 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNA silencing of single and multiple members in a gene family of rice.

Plant physiology ·Vol. 138 ·No. 4 ·2005-08-00 ·Pages 1903-13

Miki D, Itoh R, Shimamoto K

Abstract

RNA silencing with inverted repeat (IR) constructs has been used to suppress gene expression in various organisms. However, the transitive RNA-silencing effect described in plants may preclude the use of RNA silencing for a gene family. Here, we show that, in rice (Oryza sativa), transitive RNA silencing (spreading of double-stranded RNA along the target mRNA) occurred with the green fluorescent protein transgene but not with the endogenous phytoene desaturase gene. We fused IR copies of unique 3' untranslated regions derived from the rice OsRac gene family to a strong promoter and stably introduced them into rice. Each of the seven members of the OsRac gene family was specifically suppressed by its respective IR construct. We also examined IR constructs in which multiple 3' untranslated regions were fused and showed that three members of the OsRac gene family were effectively suppressed by a single construct. Using highly conserved regions of the two members of the OsRac gene family, we also suppressed the expression of all members of the gene family with variable efficiencies. These results suggest that RNA silencing is a useful method for the functional analysis of gene families in rice and other plants.

MeSH Terms
3' Untranslated Regions Conserved Sequence Gene Expression Regulation, Plant/physiology Multigene Family/physiology Oryza/genetics,metabolism Oxidoreductases/biosynthesis RNA Interference RNA, Plant RNA, Small Interfering/classification,metabolism Repetitive Sequences, Nucleic Acid rac GTP-Binding Proteins/biosynthesis
Chemicals
3' Untranslated Regions RNA, Plant RNA, Small Interfering Oxidoreductases phytoene dehydrogenase rac GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miki Daisuke
Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
Itoh Rika
Shimamoto Ko
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.105.063933
Published
2005-08-00
Pages
1903-13
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1183382
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.105.063933 PMC Full Text View journal details
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