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PMID: 16151097 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

New chitosan-degrading strains that produce chitosanases similar to ChoA of Mitsuaria chitosanitabida.

Applied and environmental microbiology ·Vol. 71 ·No. 9 ·2005-09-00 ·Pages 5138-44

Yun C, Amakata D, Matsuo Y, Matsuda H, Kawamukai M

Abstract

The betaproteobacterium Mitsuaria chitosanitabida (formerly Matsuebacter chitosanotabidus) 3001 produces a chitosanase (ChoA) that is classified in glycosyl hydrolase family 80. While many chitosanase genes have been isolated from various bacteria to date, they show limited homology to the M. chitosanitabida 3001 chitosanase gene (choA). To investigate the phylogenetic distribution of chitosanases analogous to ChoA in nature, we identified 67 chitosan-degrading strains by screening and investigated their physiological and biological characteristics. We then searched for similarities to ChoA by Western blotting and Southern hybridization and selected 11 strains whose chitosanases showed the most similarity to ChoA. PCR amplification and sequencing of the chitosanase genes from these strains revealed high deduced amino acid sequence similarities to ChoA ranging from 77% to 99%. Analysis of the 16S rRNA gene sequences of the 11 selected strains indicated that they are widely distributed in the beta and gamma subclasses of Proteobacteria and the Flavobacterium group. These observations suggest that the ChoA-like chitosanases that belong to family 80 occur widely in a broad variety of bacteria.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism Betaproteobacteria/enzymology,genetics,physiology Chitosan/metabolism Flavobacterium/enzymology,genetics Gammaproteobacteria/enzymology,genetics Glycoside Hydrolases/chemistry,genetics,metabolism Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Chemicals
Bacterial Proteins RNA, Ribosomal, 16S Chitosan Glycoside Hydrolases chitosanase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yun ChoongSoo
Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan.
Amakata Daiki
Matsuo Yasuhiro
Matsuda Hideyuki
Kawamukai Makoto
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.71.9.5138-5144.2005
Published
2005-09-00
Pages
5138-44
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC1214613
Subset
IM
Databases
GENBANK
AB024305, AB024306, AB024308, AY856841, AY856842, AY856843, AY856844, AY856845, AY856846, AY856847, AY856848, AY856849, AY856850, AY856851, AY856852, AY856853, AY856854, AY856855, AY856856, AY856857, AY856858, AY856859
External Links
PubMed source DOI: 10.1128/AEM.71.9.5138-5144.2005 PMC Full Text View journal details
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