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PMID: 11388486 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660.

Bioscience, biotechnology, and biochemistry ·Vol. 65 ·No. 4 ·2001-04-00 ·Pages 977-81

Zhang XY, Dai AL, Kuroiwa K, Kodaira R, Nogawa M, Shimosaka M, Okazaki M

Abstract

A genomic copy of the gene coding for chitosanase (csnA) was isolated from Aspergillus oryzae IAM 2660. A. oryzae csnA contains an open reading frame that encodes a polypeptide of 245 amino acids with a calculated molecular mass of 26,500 Da. The deduced amino acid sequence of A. oryzae csnA indicates extensive similarities to those of other fungal chitosanases.

MeSH Terms

Amino Acid Sequence Aspergillus fumigatus/enzymology,genetics Aspergillus oryzae/enzymology,genetics Cloning, Molecular Fusarium/enzymology,genetics Gene Library Glycoside Hydrolases/genetics Molecular Sequence Data Reverse Transcriptase Polymerase Chain Reaction Species Specificity

Chemicals

Glycoside Hydrolases chitosanase

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Zhang X Y

Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.

Dai A L

Kuroiwa K

Kodaira R

Nogawa M

Shimosaka M

Okazaki M

Article Info

Journal

Bioscience, biotechnology, and biochemistry

Abbr.

Biosci Biotechnol Biochem

ISSN

0916-8451

DOI

10.1271/bbb.65.977

Published

2001-04-00

Pages

977-81

Language

English

Region

England

NLM ID

9205717

Subset

Databases

GENBANK

AF105078

External Links

PubMed source DOI: 10.1271/bbb.65.977 View journal details

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Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660.

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