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PMID: 1614867 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoter specificity of the two transcriptional activation functions of the human oestrogen receptor in yeast.

Nucleic acids research ·Vol. 20 ·No. 11 ·1992-06-11 ·Pages 2813-7

Metzger D, Losson R, Bornert JM, Lemoine Y, Chambon P

Abstract

We have previously demonstrated that the human oestrogen receptor (hER) contains two transcriptional activation functions located in the N-terminal region (TAF-1) and in the hormone binding domain (TAF-2), which can act both independently and synergistically in a promoter- and cell-specific manner in animal cells. We have also demonstrated that hER can activate transcription from chimaeric oestrogen-responsive GAL1 promoters in yeast, and shown that transcriptional activation was due to TAF-1, whereas TAF-2 showed little, if any, transcriptional activity on these promoters. By using a more complex promoter derived from the URA3 gene, we now show that TAF-2 is also active in yeast, and that the activities of TAF-1 and TAF-2 are promoter-context-specific in yeast. We also confirm that the agonistic activity of 4-hydroxytamoxifen (OHT) can be ascribed to the activity of TAF-1.

MeSH Terms
Base Sequence Estrogens/pharmacology Gene Expression Regulation, Fungal/drug effects Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Promoter Regions, Genetic Receptors, Estrogen/genetics Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Tamoxifen/analogs & derivatives,pharmacology Transcription Factors/genetics Transcription, Genetic/drug effects
Chemicals
Estrogens Oligodeoxyribonucleotides Receptors, Estrogen Transcription Factors Tamoxifen afimoxifene
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Metzger D
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Strasburg, France.
Losson R
Bornert J M
Lemoine Y
Chambon P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.11.2813
Published
1992-06-11
Pages
2813-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336926
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.11.2813 PMC Full Text View journal details
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