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PMID: 1614819 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inactivation of the Ba2+ current in dissociated Helix neurons: voltage dependence and the role of phosphorylation.

Pflugers Archiv : European journal of physiology ·Vol. 420 ·No. 5-6 ·1992-04-00 ·Pages 470-8

Yakel JL

Abstract

The rate of inactivation of the voltage-dependent Ba2+ current in dissociated neurons from the snail Helix aspersa was found to be modulated by phosphorylation. Conditions were chosen such that the most likely mechanism of inactivation of the Ba2+ current was a voltage-dependent/calcium-independent inactivation process. If adenosine-triphosphate (ATP) was not included in the patch electrode filling solution, or if alkaline phosphatase was added, the Ba2+ current rapidly ran down and the rate of inactivation greatly increased with time. Dialysis with either ATP gamma S or the phosphatase inhibitor okadaic acid (OA) either enhanced the amplitude or greatly reduced the rate of run-down of the Ba2+ current (depending upon the presence of ATP), as well as reducing the rate of inactivation. However, dialysis with either the catalytic subunit of the cyclic-adenosine-mono-phosphate-dependent protein kinase (cAMP-PK), a synthetic peptide inhibitor of this enzyme, or staurosporine (a potent inhibitor of protein kinase C), did not have any significant effect on the amplitude or kinetics of the Ba2+ current. Surprisingly, dialysis with a peptide inhibitor (CKIP) of the Ca2+/calmodulin-dependent protein kinase II (Ca(2+)-CaM-PK) significantly reduced the rate of inactivation of this current. These results suggest that phosphorylation may exert its effect by modulating the gating properties of the Ca2+ channels.

MeSH Terms
Adenosine Triphosphate/pharmacology Animals Barium/physiology Helix, Snails Neurons/physiology Phosphorylation
Chemicals
Barium Adenosine Triphosphate
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Yakel J L
Laboratoire de Neurobiologie (URA 295 CNRS), Ecole Normale Supérieure, Paris, France.
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00374621
Published
1992-04-00
Pages
470-8
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00374621 View journal details
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