Home LiteratureArticle Details
PMID: 16107562 Published · ppublish English Journal Article

A role for Fis1 in both mitochondrial and peroxisomal fission in mammalian cells.

Molecular biology of the cell ·Vol. 16 ·No. 11 ·2005-11-00 ·Pages 5077-86

Koch A, Yoon Y, Bonekamp NA, McNiven MA, Schrader M

Abstract

The mammalian dynamin-like protein DLP1/Drp1 has been shown to mediate both mitochondrial and peroxisomal fission. In this study, we have examined whether hFis1, a mammalian homologue of yeast Fis1, which has been shown to participate in mitochondrial fission by an interaction with DLP1/Drp1, is also involved in peroxisomal growth and division. We show that hFis1 localizes to peroxisomes in addition to mitochondria. Through differential tagging and deletion experiments, we demonstrate that the transmembrane domain and the short C-terminal tail of hFis1 is both necessary and sufficient for its targeting to peroxisomes and mitochondria, whereas the N-terminal region is required for organelle fission. hFis1 promotes peroxisome division upon ectopic expression, whereas silencing of Fis1 by small interfering RNA inhibited fission and caused tubulation of peroxisomes. These findings provide the first evidence for a role of Fis1 in peroxisomal fission and suggest that the fission machinery of mitochondria and peroxisomes shares common components.

MeSH Terms
Animals COS Cells Cell Line, Tumor Chlorocebus aethiops Dynamins GTP Phosphohydrolases/metabolism Humans Membrane Proteins/chemistry,metabolism,physiology Microtubule-Associated Proteins/metabolism Mitochondria/metabolism Mitochondrial Proteins/metabolism,physiology Peroxisomes/metabolism RNA, Small Interfering/pharmacology Rats Transfection
Chemicals
FIS1 protein, human Membrane Proteins Microtubule-Associated Proteins Mitochondrial Proteins RNA, Small Interfering GTP Phosphohydrolases DNM1L protein, human Dynamins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Koch Annett
Department of Cell Biology and Cell Pathology, University of Marburg, 35037 Marburg, Germany.
Yoon Yisang
Bonekamp Nina A
McNiven Mark A
Schrader Michael
References (41)
41 references, click to expand
  1. Dynamin-like protein 1 is involved in peroxisomal fission.
    J Biol Chem. 2003 Mar 7;278(10):8597-605 PMID: 12499366
  2. The Candida boidinii peroxisomal membrane protein Pmp30 has a role in peroxisomal proliferation and is functionally homologous to Pmp27 from Saccharomyces cerevisiae.
    J Bacteriol. 1995 Dec;177(23):6773-81 PMID: 7592467
  3. The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast.
    Nat Cell Biol. 1999 Sep;1(5):298-304 PMID: 10559943
  4. The dynamin-like protein DLP1 is essential for normal distribution and morphology of the endoplasmic reticulum and mitochondria in mammalian cells.
    Mol Biol Cell. 1999 Dec;10(12):4403-17 PMID: 10588666
  5. C. elegans dynamin-related protein DRP-1 controls severing of the mitochondrial outer membrane.
    Mol Cell. 1999 Nov;4(5):815-26 PMID: 10619028
  6. Real time imaging reveals a peroxisomal reticulum in living cells.
    J Cell Sci. 2000 Oct;113 ( Pt 20):3663-71 PMID: 11017881
  7. Gag3p, an outer membrane protein required for fission of mitochondrial tubules.
    J Cell Biol. 2000 Oct 16;151(2):333-40 PMID: 11038180
  8. Mdv1p is a WD repeat protein that interacts with the dynamin-related GTPase, Dnm1p, to trigger mitochondrial division.
    J Cell Biol. 2000 Oct 16;151(2):353-66 PMID: 11038182
  9. Dnm1p GTPase-mediated mitochondrial fission is a multi-step process requiring the novel integral membrane component Fis1p.
    J Cell Biol. 2000 Oct 16;151(2):367-80 PMID: 11038183
  10. Dynamin-related protein Drp1 is required for mitochondrial division in mammalian cells.
    Mol Biol Cell. 2001 Aug;12(8):2245-56 PMID: 11514614
  11. Mammalian dynamin-like protein DLP1 tubulates membranes.
    Mol Biol Cell. 2001 Sep;12(9):2894-905 PMID: 11553726
  12. A single gene produces mitochondrial, cytoplasmic, and peroxisomal NADP-dependent isocitrate dehydrogenase in Aspergillus nidulans.
    J Biol Chem. 2001 Oct 5;276(40):37722-9 PMID: 11483612
  13. Peroxisome biogenesis.
    Annu Rev Cell Dev Biol. 2001;17:701-52 PMID: 11687502
  14. A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae.
    J Cell Biol. 2001 Dec 10;155(6):979-90 PMID: 11733545
  15. PEX11 beta deficiency is lethal and impairs neuronal migration but does not abrogate peroxisome function.
    Mol Cell Biol. 2002 Jun;22(12):4358-65 PMID: 12024045
  16. The WD repeat protein, Mdv1p, functions as a molecular adaptor by interacting with Dnm1p and Fis1p during mitochondrial fission.
    J Cell Biol. 2002 Aug 5;158(3):445-52 PMID: 12163467
  17. Alanine:glyoxylate aminotransferase peroxisome-to-mitochondrion mistargeting in human hereditary kidney stone disease.
    Biochim Biophys Acta. 2003 Apr 11;1647(1-2):70-5 PMID: 12686111
  18. The dynamin-like GTPase DLP1 is essential for peroxisome division and is recruited to peroxisomes in part by PEX11.
    J Biol Chem. 2003 May 9;278(19):17012-20 PMID: 12618434
  19. Peroxisomal motility and interaction with microtubules.
    Microsc Res Tech. 2003 Jun 1;61(2):171-8 PMID: 12740823
  20. The mitochondrial protein hFis1 regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1.
    Mol Cell Biol. 2003 Aug;23(15):5409-20 PMID: 12861026
  21. hFis1, a novel component of the mammalian mitochondrial fission machinery.
    J Biol Chem. 2003 Sep 19;278(38):36373-9 PMID: 12783892
  22. The solution structure of human mitochondria fission protein Fis1 reveals a novel TPR-like helix bundle.
    J Mol Biol. 2003 Nov 28;334(3):445-58 PMID: 14623186
  23. Cytosolic domain of the human mitochondrial fission protein fis1 adopts a TPR fold.
    Proteins. 2004 Jan 1;54(1):153-6 PMID: 14705031
  24. Levels of human Fis1 at the mitochondrial outer membrane regulate mitochondrial morphology.
    J Cell Sci. 2004 Mar 1;117(Pt 7):1201-10 PMID: 14996942
  25. The dynamin superfamily: universal membrane tubulation and fission molecules?
    Nat Rev Mol Cell Biol. 2004 Feb;5(2):133-47 PMID: 15040446
  26. An Arabidopsis dynamin-related protein, DRP3A, controls both peroxisomal and mitochondrial division.
    Plant J. 2004 May;38(3):487-98 PMID: 15086806
  27. Interaction of microtubules with peroxisomes. Tubular and spherical peroxisomes in HepG2 cells and their alterations induced by microtubule-active drugs.
    Eur J Cell Biol. 1996 Jan;69(1):24-35 PMID: 8825021
  28. A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells.
    J Cell Biol. 1998 Feb 23;140(4):779-93 PMID: 9472031
  29. Peroxisome biogenesis: involvement of ARF and coatomer.
    J Cell Biol. 1998 Apr 20;141(2):373-83 PMID: 9548716
  30. Elongation and clustering of glycosomes in Trypanosoma brucei overexpressing the glycosomal Pex11p.
    EMBO J. 1998 Jul 1;17(13):3542-55 PMID: 9649425
  31. The dynamin-related GTPase, Dnm1p, controls mitochondrial morphology in yeast.
    J Cell Biol. 1998 Oct 19;143(2):333-49 PMID: 9786946
  32. Expression of PEX11beta mediates peroxisome proliferation in the absence of extracellular stimuli.
    J Biol Chem. 1998 Nov 6;273(45):29607-14 PMID: 9792670
  33. Peroxisomal membrane proteins contain common Pex19p-binding sites that are an integral part of their targeting signals.
    Mol Biol Cell. 2004 Jul;15(7):3406-17 PMID: 15133130
  34. Peroxisome elongation and constriction but not fission can occur independently of dynamin-like protein 1.
    J Cell Sci. 2004 Aug 1;117(Pt 17):3995-4006 PMID: 15286177
  35. Identity of long-chain acyl-coenzyme A synthetase of microsomes, mitochondria, and peroxisomes in rat liver.
    J Biochem. 1985 Sep;98(3):723-33 PMID: 3936842
  36. Biogenesis of peroxisomes.
    Annu Rev Cell Biol. 1985;1:489-530 PMID: 3916321
  37. Selective induction of peroxisomal enzymes by the hypolipidemic drug bezafibrate. Detection of modulations by automatic image analysis in conjunction with immunoelectron microscopy and immunoblotting.
    Eur J Cell Biol. 1988 Aug;46(3):383-93 PMID: 2903052
  38. Biochemistry of peroxisomes.
    Annu Rev Biochem. 1992;61:157-97 PMID: 1353950
  39. Giant peroxisomes in oleic acid-induced Saccharomyces cerevisiae lacking the peroxisomal membrane protein Pmp27p.
    J Cell Biol. 1995 Feb;128(4):509-23 PMID: 7860627
  40. Pmp27 promotes peroxisomal proliferation.
    J Cell Biol. 1995 Apr;129(2):345-55 PMID: 7721939
  41. Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape.
    J Cell Biol. 1999 Nov 15;147(4):699-706 PMID: 10562274
Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e05-02-0159
Published
2005-11-00
Epub
2005-00-17
Pages
5077-86
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1266408
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e05-02-0159 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2