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PMID: 1610193 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis.

Applied and environmental microbiology ·Vol. 58 ·No. 2 ·1992-02-00 ·Pages 709-12

Howard PJ, Harsono KD, Luchansky JB

Abstract

Clamped homogeneous electric field analysis of Listeria DNA with ApaI, AscI, SmaI, or NotI revealed species- and serotype-specific differences in genomic fingerprints. Clamped homogeneous electric field analysis may prove useful, therefore, in epidemiologic studies. Also, the summation of individually sized AscI fragments of genomic DNA from L. monocytogenes serotype 4b 101M and Scott A indicated genome lengths of 2,925 and 3,046 kb, respectively.

MeSH Terms
DNA Fingerprinting Electrophoresis, Gel, Pulsed-Field Genome, Bacterial Listeria/classification,genetics,isolation & purification Listeria monocytogenes/classification,genetics,isolation & purification Serotyping
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Howard P J
Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706-1187.
Harsono K D
Luchansky J B
References (17)
17 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.58.2.709-712.1992
Published
1992-02-00
Pages
709-12
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC195308
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.58.2.709-712.1992 PMC Full Text View journal details
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