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PMID: 2125817 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Detection of Listeria monocytogenes by using the polymerase chain reaction.

Applied and environmental microbiology ·Vol. 56 ·No. 9 ·1990-09-00 ·Pages 2930-2

Bessesen MT, Luo QA, Rotbart HA, Blaser MJ, Ellison RT

Abstract

A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.

MeSH Terms
Bacteria/isolation & purification Evaluation Studies as Topic Humans Listeria/isolation & purification Listeria monocytogenes/genetics,isolation & purification,pathogenicity Polymerase Chain Reaction/methods,statistics & numerical data Sensitivity and Specificity Species Specificity
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bessesen M T
Medical Service, Department of Veterans Affairs Medical Center, Denver, Colorado.
Luo Q A
Rotbart H A
Blaser M J
Ellison R T
References (10)
10 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.56.9.2930-2932.1990
Published
1990-09-00
Pages
2930-2
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC184869
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.56.9.2930-2932.1990 PMC Full Text View journal details
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