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PMID: 16079915 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.

The EMBO journal ·Vol. 24 ·No. 16 ·2005-08-17 ·Pages 2931-43

Sanchez-Perez I, Renwick SJ, Crawley K, Karig I, Buck V, Meadows JC, Franco-Sanchez A, Fleig U, Toda T, Millar JB

Abstract

We identified a truncated allele of dam1 as a multicopy suppressor of the sensitivity of cdc13-117 (cyclin B) and mal3-1 (EB-1) cells to thiabendazole, a microtubule poison. We find that Dam1 binds to the plus end of spindle microtubules and kinetochores as cells enter mitosis and this is dependent on other components of the fission yeast DASH complex, including Ask1, Duo1, Spc34 and Dad1. By contrast, Dad1 remains bound to kinetochores throughout the cell cycle and its association is dependent on the Mis6 and Mal2, but not Mis12, Nuf2 or Cnp1, kinetochore proteins. In cells lacking Dam1, or other components of the DASH complex, anaphase is delayed due to activation of the spindle assembly checkpoint and lagging sister chromatids are frequently observed and occasionally sister chromatid pairs segregate to the same spindle pole. We find that the mitotic centromere-associated Klp5/Klp6 kinesin complex is essential in cells lacking components of the DASH complex. Cells lacking both Dam1 and Klp5 undergo a first cell cycle arrest in mitosis due to a failure to establish bipolar chromosome attachment.

MeSH Terms
Chromosome Segregation/physiology Chromosomes, Fungal/metabolism,physiology Cloning, Molecular Cyclin B DNA Primers Genes, Suppressor/physiology Kinesins/genetics,metabolism Macromolecular Substances/metabolism Microscopy, Fluorescence Microtubule-Associated Proteins/genetics,metabolism Mitosis/physiology Schizosaccharomyces Schizosaccharomyces pombe Proteins/genetics,metabolism Thiabendazole
Chemicals
Cyclin B DNA Primers Macromolecular Substances Microtubule-Associated Proteins Schizosaccharomyces pombe Proteins Klp5 protein, S pombe Klp6 protein, S pombe Kinesins Thiabendazole
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Sanchez-Perez Isabel
Division of Yeast Genetics, National Institute for Medical Research, London, UK.
Renwick Steven J
Crawley Karen
Karig Inga
Buck Vicky
Meadows John C
Franco-Sanchez Alejandro
Fleig Ursula
Toda Takashi
Millar Jonathan B A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600761
Published
2005-08-17
Epub
2005-00-04
Pages
2931-43
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1187944
Subset
IM
Grants
Medical Research Council · MC_U117531948 · United Kingdom
External Links
PubMed source DOI: 10.1038/sj.emboj.7600761 PMC Full Text View journal details
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