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PMID: 11018050 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tea2p is a kinesin-like protein required to generate polarized growth in fission yeast.

The Journal of cell biology ·Vol. 151 ·No. 1 ·2000-10-02 ·Pages 15-28

Browning H, Hayles J, Mata J, Aveline L, Nurse P, McIntosh JR

Abstract

Cytoplasmic microtubules are critical for establishing and maintaining cell shape and polarity. Our investigations of kinesin-like proteins (klps) and morphological mutants in the fission yeast Schizosaccharomyces pombe have identified a kinesin-like gene, tea2(+), that is required for cells to generate proper polarized growth. Cells deleted for this gene are often bent during exponential growth and initiate growth from improper sites as they exit stationary phase. They have a reduced cytoplasmic microtubule network and display severe morphological defects in genetic backgrounds that produce long cells. The tip-specific marker, Tea1p, is mislocalized in both tea2-1 and tea2Delta cells, indicating that Tea2p function is necessary for proper localization of Tea1p. Tea2p is localized to the tips of the cell and in a punctate pattern within the cell, often coincident with the ends of cytoplasmic microtubules. These results suggest that this kinesin promotes microtubule growth, possibly through interactions with the microtubule end, and that it is important for establishing and maintaining polarized growth along the long axis of the cell.

MeSH Terms
Amino Acid Sequence Cell Compartmentation Cell Polarity/genetics Cloning, Molecular Fungal Proteins/genetics Genes, Fungal Microtubule-Associated Proteins/genetics Microtubules/ultrastructure Molecular Motor Proteins Molecular Sequence Data Protein Biosynthesis Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Schizosaccharomyces/cytology,genetics,growth & development Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Fungal Proteins KIP2 protein, S cerevisiae Microtubule-Associated Proteins Molecular Motor Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Tea2 protein, S pombe
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Browning H
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA. browninh@icrf.icnet.uk
Hayles J
Mata J
Aveline L
Nurse P
McIntosh J R
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.151.1.15
Email
browninh@icrf.icnet.uk
Published
2000-10-02
Pages
15-28
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2189814
Subset
IM
Grants
NIGMS NIH HHS · GM17117 · United States
NIGMS NIH HHS · GM36663 · United States
Databases
GENBANK
AL034433
External Links
PubMed source DOI: 10.1083/jcb.151.1.15 PMC Full Text View journal details
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