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PMID: 16061805 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Cardiac repair with intramyocardial injection of allogeneic mesenchymal stem cells after myocardial infarction.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 32 ·2005-08-09 ·Pages 11474-9

Amado LC, Saliaris AP, Schuleri KH, St John M, Xie JS, Cattaneo S, Durand DJ, Fitton T, Kuang JQ, Stewart G, Lehrke S, Baumgartner WW, Martin BJ, Heldman AW, Hare JM

Abstract

Although clinical trials of autologous whole bone marrow for cardiac repair demonstrate promising results, many practical and mechanistic issues regarding this therapy remain highly controversial. Here, we report the results of a randomized study of bone-marrow-derived mesenchymal stem cells, administered to pigs, which offer several new insights regarding cellular cardiomyoplasty. First, cells were safely injected by using a percutaneous-injection catheter 3 d after myocardial infarction. Second, cellular transplantation resulted in long-term engraftment, profound reduction in scar formation, and near-normalization of cardiac function. Third, transplanted cells were pre-prepared from an allogeneic donor and were not rejected, a major practical advance for widespread application of this therapy. Together, these findings demonstrate that the direct injection of cellular grafts into damaged myocardium is safe and effective in the perii-nfarct period. The direct delivery of cells to necrotic myocardium offers a valuable alternative to intracoronary cell injections, and the use of allogeneic mesenchymal stem cells provides a valuable strategy for cardiac regenerative therapy that avoids the need for preparing autologous cells from the recipient.

MeSH Terms
Analysis of Variance Animals Cardiac Catheterization Female Immunohistochemistry Injections Magnetic Resonance Imaging Mesenchymal Stem Cell Transplantation/methods Myocardial Contraction/physiology Myocardial Infarction/pathology,therapy Myocytes, Cardiac/physiology Regeneration/physiology Sus scrofa Transplantation, Homologous
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Amado Luciano C
Department of Medicine, Cardiology Division, The Johns Hopkins Hospital, Blalock 618, 600 North Wolfe Street, Baltimore, MD 21287, USA.
Saliaris Anastasios P
Schuleri Karl H
St John Marcus
Xie Jin-Sheng
Cattaneo Stephen
Durand Daniel J
Fitton Torin
Kuang Jin Qiang
Stewart Garrick
Lehrke Stephanie
Baumgartner William W
Martin Bradley J
Heldman Alan W
Hare Joshua M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0504388102
Published
2005-08-09
Epub
2005-00-01
Pages
11474-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1183573
Subset
IM
Grants
NIA NIH HHS · R01 AG025017 · United States
NHLBI NIH HHS · R21 HL072185 · United States
NIA NIH HHS · R01 NIA AG025017 · United States
NHLBI NIH HHS · R21 HL-72185 · United States
External Links
PubMed source DOI: 10.1073/pnas.0504388102 PMC Full Text View journal details
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