Home LiteratureArticle Details
PMID: 14530411 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cardiac progenitor cells from adult myocardium: homing, differentiation, and fusion after infarction.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 21 ·2003-10-14 ·Pages 12313-8

Oh H, Bradfute SB, Gallardo TD, Nakamura T, Gaussin V, Mishina Y, Pocius J, Michael LH, Behringer RR, Garry DJ, Entman ML, Schneider MD

Abstract

Potential repair by cell grafting or mobilizing endogenous cells holds particular attraction in heart disease, where the meager capacity for cardiomyocyte proliferation likely contributes to the irreversibility of heart failure. Whether cardiac progenitors exist in adult myocardium itself is unanswered, as is the question whether undifferentiated cardiac precursor cells merely fuse with preexisting myocytes. Here we report the existence of adult heart-derived cardiac progenitor cells expressing stem cell antigen-1. Initially, the cells express neither cardiac structural genes nor Nkx2.5 but differentiate in vitro in response to 5'-azacytidine, in part depending on Bmpr1a, a receptor for bone morphogenetic proteins. Given intravenously after ischemia/reperfusion, cardiac stem cell antigen 1 cells home to injured myocardium. By using a Cre/Lox donor/recipient pair (alphaMHC-Cre/R26R), differentiation was shown to occur roughly equally, with and without fusion to host cells.

MeSH Terms
Animals Cell Differentiation Cell Fusion Cell Movement Cell Separation Gene Expression Genes Genes, Reporter In Vitro Techniques Mice Mice, Inbred C57BL Myoblasts, Cardiac/metabolism,pathology Myocardial Infarction/genetics,pathology Transcription Factors/genetics
Chemicals
Transcription Factors
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Oh Hidemasa
Center for Cardiovascular Development, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Bradfute Steven B
Gallardo Teresa D
Nakamura Teruya
Gaussin Vinciane
Mishina Yuji
Pocius Jennifer
Michael Lloyd H
Behringer Richard R
Garry Daniel J
Entman Mark L
Schneider Michael D
References (37)
37 references, click to expand
  1. Pluripotency of mesenchymal stem cells derived from adult marrow.
    Nature. 2002 Jul 4;418(6893):41-9 PMID: 12077603
  2. Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts.
    Circulation. 2002 Jul 2;106(1):17-9 PMID: 12093763
  3. Little evidence for developmental plasticity of adult hematopoietic stem cells.
    Science. 2002 Sep 27;297(5590):2256-9 PMID: 12215650
  4. Repair of infarcted myocardium by autologous intracoronary mononuclear bone marrow cell transplantation in humans.
    Circulation. 2002 Oct 8;106(15):1913-8 PMID: 12370212
  5. Myogenic specification of side population cells in skeletal muscle.
    J Cell Biol. 2002 Oct 14;159(1):123-34 PMID: 12379804
  6. "Stemness": transcriptional profiling of embryonic and adult stem cells.
    Science. 2002 Oct 18;298(5593):597-600 PMID: 12228720
  7. Side population cells from diverse adult tissues are capable of in vitro hematopoietic differentiation.
    Exp Hematol. 2002 Nov;30(11):1339-45 PMID: 12423688
  8. Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction (TOPCARE-AMI).
    Circulation. 2002 Dec 10;106(24):3009-17 PMID: 12473544
  9. Transdifferentiation of blood-derived human adult endothelial progenitor cells into functionally active cardiomyocytes.
    Circulation. 2003 Feb 25;107(7):1024-32 PMID: 12600917
  10. Autologous skeletal myoblast transplantation for severe postinfarction left ventricular dysfunction.
    J Am Coll Cardiol. 2003 Apr 2;41(7):1078-83 PMID: 12679204
  11. Cell fusion is the principal source of bone-marrow-derived hepatocytes.
    Nature. 2003 Apr 24;422(6934):897-901 PMID: 12665832
  12. Transplanted bone marrow regenerates liver by cell fusion.
    Nature. 2003 Apr 24;422(6934):901-4 PMID: 12665833
  13. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo.
    J Exp Med. 1996 Apr 1;183(4):1797-806 PMID: 8666936
  14. Cardiomyocyte DNA synthesis and binucleation during murine development.
    Am J Physiol. 1996 Nov;271(5 Pt 2):H2183-9 PMID: 8945939
  15. Gene recombination in postmitotic cells. Targeted expression of Cre recombinase provokes cardiac-restricted, site-specific rearrangement in adult ventricular muscle in vivo.
    J Clin Invest. 1997 Jul 1;100(1):169-79 PMID: 9202069
  16. Generalized lacZ expression with the ROSA26 Cre reporter strain.
    Nat Genet. 1999 Jan;21(1):70-1 PMID: 9916792
  17. Cardiomyocytes can be generated from marrow stromal cells in vitro.
    J Clin Invest. 1999 Mar;103(5):697-705 PMID: 10074487
  18. Bone morphogenetic proteins induce cardiomyocyte differentiation through the mitogen-activated protein kinase kinase kinase TAK1 and cardiac transcription factors Csx/Nkx-2.5 and GATA-4.
    Mol Cell Biol. 1999 Oct;19(10):7096-105 PMID: 10490646
  19. Hematopoietic potential of stem cells isolated from murine skeletal muscle.
    Proc Natl Acad Sci U S A. 1999 Dec 7;96(25):14482-6 PMID: 10588731
  20. A chronic mouse model of myocardial ischemia-reperfusion: essential in cytokine studies.
    Am J Physiol Heart Circ Physiol. 2000 Apr;278(4):H1049-55 PMID: 10749697
  21. Transplanted cord blood-derived endothelial precursor cells augment postnatal neovascularization.
    J Clin Invest. 2000 Jun;105(11):1527-36 PMID: 10841511
  22. Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology.
    Am J Physiol Heart Circ Physiol. 2000 Jul;279(1):H429-36 PMID: 10899083
  23. Intraarterial injection of muscle-derived CD34(+)Sca-1(+) stem cells restores dystrophin in mdx mice.
    J Cell Biol. 2001 Jan 22;152(2):335-48 PMID: 11266450
  24. Can stem cells cross lineage boundaries?
    Nat Med. 2001 Apr;7(4):393-5 PMID: 11283651
  25. Bone marrow cells regenerate infarcted myocardium.
    Nature. 2001 Apr 5;410(6829):701-5 PMID: 11287958
  26. Evidence that human cardiac myocytes divide after myocardial infarction.
    N Engl J Med. 2001 Jun 7;344(23):1750-7 PMID: 11396441
  27. Regeneration of ischemic cardiac muscle and vascular endothelium by adult stem cells.
    J Clin Invest. 2001 Jun;107(11):1395-402 PMID: 11390421
  28. The evolving concept of a stem cell: entity or function?
    Cell. 2001 Jun 29;105(7):829-41 PMID: 11439179
  29. Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply of angioblasts, angiogenic ligands, and cytokines.
    Circulation. 2001 Aug 28;104(9):1046-52 PMID: 11524400
  30. Mobilized bone marrow cells repair the infarcted heart, improving function and survival.
    Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10344-9 PMID: 11504914
  31. Telomerase reverse transcriptase promotes cardiac muscle cell proliferation, hypertrophy, and survival.
    Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10308-13 PMID: 11517337
  32. Cardiomyocytes induce endothelial cells to trans-differentiate into cardiac muscle: implications for myocardium regeneration.
    Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10733-8 PMID: 11535818
  33. The potential of muscle stem cells.
    Dev Cell. 2001 Sep;1(3):333-42 PMID: 11702945
  34. Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart.
    Circulation. 2002 Jan 1;105(1):93-8 PMID: 11772882
  35. Muscle-derived hematopoietic stem cells are hematopoietic in origin.
    Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1341-6 PMID: 11830662
  36. Endocardial cushion and myocardial defects after cardiac myocyte-specific conditional deletion of the bone morphogenetic protein receptor ALK3.
    Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2878-83 PMID: 11854453
  37. Failure of bone marrow cells to transdifferentiate into neural cells in vivo.
    Science. 2002 Aug 23;297(5585):1299 PMID: 12193778
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.2132126100
Published
2003-10-14
Epub
2003-00-06
Pages
12313-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC218755
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.2132126100 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2