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PMID: 16030221 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cystic fibrosis sputum supports growth and cues key aspects of Pseudomonas aeruginosa physiology.

Journal of bacteriology ·Vol. 187 ·No. 15 ·2005-08-00 ·Pages 5267-77

Palmer KL, Mashburn LM, Singh PK, Whiteley M

Abstract

The opportunistic human pathogen Pseudomonas aeruginosa causes persistent airway infections in patients with cystic fibrosis (CF). To establish these chronic infections, P. aeruginosa must grow and proliferate within the highly viscous sputum in the lungs of CF patients. In this study, we used Affymetrix GeneChip microarrays to investigate the physiology of P. aeruginosa grown using CF sputum as the sole source of carbon and energy. Our results indicate that CF sputum readily supports high-density P. aeruginosa growth. Furthermore, multiple signals, which reduce swimming motility and prematurely activate the Pseudomonas quinolone signal cell-to-cell signaling cascade in P. aeruginosa, are present in CF sputum. P. aeruginosa factors critical for lysis of the common CF lung inhabitant Staphylococcus aureus were also induced in CF sputum and increased the competitiveness of P. aeruginosa during polymicrobial growth in CF sputum.

MeSH Terms
Culture Media Cystic Fibrosis/microbiology Humans Locomotion Pseudomonas aeruginosa/growth & development,physiology Sputum
Chemicals
Culture Media
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Palmer Kelli L
Department of Periodontics, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Mashburn Lauren M
Singh Pradeep K
Whiteley Marvin
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.187.15.5267-5277.2005
Published
2005-08-00
Pages
5267-77
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1196007
Subset
IM
Grants
NCRR NIH HHS · P20 RR015564 · United States
NCRR NIH HHS · 1P20RR1556401 · United States
External Links
PubMed source DOI: 10.1128/JB.187.15.5267-5277.2005 PMC Full Text View journal details
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