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PMID: 10648542 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Crc is involved in catabolite repression control of the bkd operons of Pseudomonas putida and Pseudomonas aeruginosa.

Journal of bacteriology ·Vol. 182 ·No. 4 ·2000-02-00 ·Pages 1144-9

Hester KL, Lehman J, Najar F, Song L, Roe BA, MacGregor CH, Hager PW, Phibbs PV, Sokatch JR

Abstract

Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study, the role of Crc in catabolite repression control has been studied in Pseudomonas putida. The bkd operons of P. putida and P. aeruginosa encode the inducible multienzyme complex branched-chain keto acid dehydrogenase, which is regulated in both species by catabolite repression. We report here that this effect is mediated in both species by Crc. A 13-kb cloned DNA fragment containing the P. putida crc gene region was sequenced. Crc regulates the expression of branched-chain keto acid dehydrogenase, glucose-6-phosphate dehydrogenase, and amidase in both species but not urocanase, although the carbon sources responsible for catabolite repression in the two species differ. Transposon mutants affected in their expression of BkdR, the transcriptional activator of the bkd operon, were isolated and identified as crc and vacB (rnr) mutants. These mutants suggested that catabolite repression in pseudomonads might, in part, involve control of BkdR levels.

MeSH Terms
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) Amidohydrolases/genetics,metabolism Bacterial Proteins DNA Transposable Elements DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Bacterial Ketone Oxidoreductases/genetics,metabolism Leucine-Responsive Regulatory Protein Molecular Sequence Data Multienzyme Complexes/genetics,metabolism Mutagenesis Operon Plasmids/genetics Pseudomonas/enzymology,genetics Pseudomonas aeruginosa/enzymology,genetics Pseudomonas putida/enzymology,genetics Recombination, Genetic Repressor Proteins/genetics,isolation & purification,metabolism Ribonucleases/genetics,metabolism Sequence Analysis, DNA Transcription Factors Urocanate Hydratase/genetics,metabolism
Chemicals
Bacterial Proteins DNA Transposable Elements DNA-Binding Proteins Multienzyme Complexes Repressor Proteins Transcription Factors crc protein, Pseudomonas Leucine-Responsive Regulatory Protein Ketone Oxidoreductases 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide) Ribonucleases Amidohydrolases amidase Urocanate Hydratase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Hester K L
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA.
Lehman J
Najar F
Song L
Roe B A
MacGregor C H
Hager P W
Phibbs P V
Sokatch J R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.4.1144-1149.2000
Published
2000-02-00
Pages
1144-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94392
Subset
IM
Grants
NIDDK NIH HHS · DK21737 · United States
Databases
GENBANK
AC004396
External Links
PubMed source DOI: 10.1128/JB.182.4.1144-1149.2000 PMC Full Text View journal details
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